|
|
|
| |
Peptide-affinity Purified Polyclonal Antibody to CNOT4 |
|
|
| |
|
|
| |
|
| Catalog No: |
IMG-6243A |
| Contents: |
100 ug in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic. |
| Isotype: |
Rabbit Ig |
| Purification: |
Antigen Affinity Chromatography |
| Species: |
Chicken, Cow, Human, Mouse, Rat, Xenopus |
| Predicted React: |
Pufferfish |
| Host: |
Rabbit |
| Application: |
Western blot analysis: 0.05-0.1 ug/ml |
| Storage: |
Store at 4°C, stable for 6 months. For long-term storage, aliquot and store at -20°C. |
Recommended
Positive Control: |
Human skeletal muscle lysate |
|
 |
|
|
|
|
|
|
Background
CNOT4 is a 575 amino acid protein with E3 ubiquitin ligase activity, mainly involved in protein modification and protein ubiquitination. It contains a ring type zinc finger domain of the C4C4 type, an RNA recognition motif and a bipartite nuclear localization signal. Studies show that CNOT4 is a component of the CCR4-NOT (CAF1) core complex that functions as general transcription regulation complex and has both a positive as well as a negative effect on the transcription of multiple functionally unrelated genes, thus controls gene expression. CNOT4 interacts with CNOT1 via C-terminus and with E2 ubiquitin ligases via C4C4 RING domain. Reports suggest that CNOT4 gets phosphorylated in response to DNA damage on consensus sites recognized by ATM and ATR. |
Antigen
A portion of amino acids 50-100 of human CNOT4 was used as the immunogen. |
Application Notes
The amino acid sequence used as immunogen is 100% homologous in human, mouse, rat, chicken, cow, zebrafish and 88% homologous in pufferfish. |
Genebank Info (Protein)
NP_037448 |
| |
|
Western blot analysis of CNOT4 in human skeletal muscle lysate in the 1) absence and 2) presence of immunizing peptide using IMG-6243A at 0.05 ug/ml. |
|
|
|
|
|
| - |
 |
|
Reference
1. Albert, T.K. et al. Nucleic Acids Res. 28:809-817 (2000).
2. Matsuoka, S. et al. Science. 316:1160-1166 (2007).
3. Albert, T.K. et al. EMBO J. 21:355-364 (2002).
|
|
|
|
Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
|
|
|
|
|
