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Product : Antibody >> Antigen Affinity Purified , IHCProTM , Immunohistochemistry , Stem Cell Markers , Transcription Factors >> CNOT4
      
 

Peptide-affinity Purified Polyclonal Antibody to CNOT4

 
Cat. No.DescriptionFormatUnitPrice(USD)QuantityBulk Quotation
IMG-6243A
CNOT4Purified, Peptide Affinity
0.1 mg
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Catalog No: IMG-6243A
Imgenex is now a part of Novus Biologicals! All orders, billing and shipments will be received from Novus. Please contact Novus directly for any questions – novus@novusbio.com. To insure proper processing of you order, please insure that your purchase orders show Novus Biologicals as the vendor.
Contents: 100 ug antibody in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Isotype: Rabbit Ig
Purification: Antigen Affinity Chromatography was used to purify the CNOT antibody
Species: Chicken, Cow, Human, Mouse, Rat, Xenopus
Predicted React: Pufferfish
Host: Rabbit
Application: IHC (p): 10 ug/ml
Western blot analysis: 0.05-0.1 ug/ml
Storage: Store CNOT4 antibody at 4°C, stable for 6 months. For long-term storage, aliquot and store antibody at -20°C.
 

Background
CNOT4 is a 575 amino acid protein with E3 ubiquitin ligase activity, mainly involved in protein modification and protein ubiquitination. It contains a ring type zinc finger domain of the C4C4 type, an RNA recognition motif and a bipartite nuclear localization signal. Studies show that CNOT4 is a component of the CCR4-NOT (CAF1) core complex that functions as general transcription regulation complex and has both a positive as well as a negative effect on the transcription of multiple functionally unrelated genes, thus controls gene expression. CNOT4 interacts with CNOT1 via C-terminus and with E2 ubiquitin ligases via C4C4 RING domain. Reports suggest that CNOT4 gets phosphorylated in response to DNA damage on consensus sites recognized by ATM and ATR.

Antigen
A portion of amino acids 50-100 of human CNOT4 was used as the immunogen for the antibody.

Application Notes
The amino acid sequence used as immunogen for the antibody is 100% homologous in human, mouse, rat, chicken, cow, zebrafish and 88% homologous in pufferfish.

Genebank Info (Protein): NP_037448
Gene ID: 4850
 
CNOT4 Antibody WB

Western blot analysis of CNOT4 in human skeletal muscle lysate in the 1) absence and 2) presence of immunizing peptide using CNOT4 antibody at 0.05 ug/ml.

CNOT4 Antibody IHC

Formalin-fixed paraffin-embedded human prostate tissue stained with CNOT4 antibody at 10 ug/ml.

Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.

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Reference
1. Albert, T.K. et al. Nucleic Acids Res. 28:809-817 (2000).
2. Matsuoka, S. et al. Science. 316:1160-1166 (2007).
3. Albert, T.K. et al.  EMBO J. 21:355-364 (2002).


Research purposes only. Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.


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