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Polyclonal Antibody to GR (2- Glutathione reductase from S. cerevisiae) |
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| Catalog No: |
IMG-6247A |
| Contents: |
100 ug in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic. |
| Isotype: |
Rabbit IgG |
| Purification: |
Protein A Chromatography |
| Species: |
Human, S. cerevisiae |
| Host: |
Rabbit |
| Application: |
Western blot analysis: 0.5-1.0 ug/ml |
| Storage: |
Store at 4°C, stable for 6 months. For long-term storage, aliquot and store at -20°C. |
Recommended
Positive Control: |
Human Liver lysate |
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Background
Glutathione reductase (GSR or GR) is a crucial flavoenzyme in the antioxidant defense system that maintains high levels of reduced glutathione in the cytosol. It belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family and is a flavoprotein homodimer of two equal subunits with one mole of noncovalently bound FAD in each subunit. GR reduces glutathione disulphide (GSSG) to the sulfhydryl form (GSH) which is an important cellular oxidant and is used by glutathione peroxidase to detoxify hydrogen peroxide. Apart from NADPH, GR also uses FAD as a cofactor for the process. In sperm, GR and glutathione peroxidase function as antioxidant enzymes and thus may inhibit lipid peroxidation. GR is stimulated by melatonin and is reportedly irreversibly inhibited by a number of oxygen radical generating systems. |
Antigen
Source of Immunogen: Sigma (Cat# G3664). |
Genebank Info (Protein)
NP_001019265 |
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Western blot analysis of GR (2- Glutathione reductase from S. cerevisiae) in human liver lysate using IMG-6247A at 0.5 ug/ml. |
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Reference
1. Lenzi, A. et al. Hum. Reprod. 9:2044-2050 (1994).
2. Reiter, RJ. et al. Ann. N.Y. Acad. Sci. 854:410-424 (1998).
3. Wan, WY. et al. J. Mol. Biol. 286:1633-1649 (1999).
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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