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Peptide-affinity Purified Polyclonal Antibody to AXL/UFON |
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| Catalog No: |
IMG-6265A |
| Contents: |
100 ug in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic. |
| Isotype: |
Rabbit Ig |
| Purification: |
Antigen Affinity Chromatography |
| Species: |
Human, Mouse, Rat |
| Predicted React: |
Pig, Xenopus |
| Host: |
Rabbit |
| Application: |
Western blot analysis: 0.5-1.0 ug/ml |
| Storage: |
Store at 4°C, stable for 6 months. For long-term storage, aliquot and store at -20°C. |
Recommended
Positive Control: |
HUVEC cell lysate |
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Background
AXL/UFON is an 887 amino acid proto-oncogene belonging to the protein kinase super family with a protein kinase domain, two Ig-like C2-type (immunoglobulin-like) domains and two fibronectin type–III domains. Unlike other receptor tyrosine kinases, AXL represents a unique novel structure in the extracellular region that juxtaposes IgL and FNIII repeats. AXL may function as a signal transducer between specific cell types of mesodermal origin and may transduce signals from the extracellular matrix into the cytoplasm by binding to growth factors like vitamin K-dependent protein growth-arrest-specific gene 6 (GAS6) and thus form heterodimer and heterotetramer. It plays an important role in the stimulation of cell proliferation and can also mediate cell aggregation by homophilic binding. In case of filovirus infection, AXL seems to function as a cell entry factor. Studies reveal the transforming potential of AXL in patients with chronic myeloproliferative disorder or chronic myelocytic leukemia. |
Antigen
A portion of amino acids 450-500 of human AXL/UFON was used as the immunogen. |
Application Notes
The amino acid sequence used as immunogen is 100% homologous in human, mouse and rat, 94% homologus in pig and 94% homologus in xenopus. |
Genebank Info (Protein)
NP_001690 |
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Western blot analysis of AXL in HUVEC cell lysate in the 1) absence and 2) presence of immunizing peptide using IMG-6265A at 0.5 ug/ml. |
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Reference
1. Janssen J.W.G. et al. Oncogene. 6:2113-2120 (1991).
2. Liu E. et al. Proc. Nat. Acad. Sci. 85:1952-1956 (1988).
3. O'Bryan J.P. et al. Molec. Cell. Biol. 11:5016-5031 (1991).
4. Varnum B.C. et al. Nature. 373:623-626 (1995).
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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