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Peptide-affinity Purified Polyclonal Antibody to TLR4 |
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| Catalog No: |
IMG-6307A |
| Contents: |
100 ug antibody in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic. |
| Isotype: |
Rabbit Ig |
| Purification: |
Antigen Affinity Chromatography |
| Species: |
Bison, Chimpanzee, Cow, Gorilla, Horse, Human, Macaque |
| Predicted React: |
Rat, Dog |
| Host: |
Rabbit |
| Application: |
Western blot analysis: 0.1-0.5 ug/ml |
| Storage: |
Store TLR4 antibody at 4°C, stable for 6 months. For long-term storage, aliquot and store antibody at -20°C. |
Recommended
Positive Control: |
Human intestine lysate |
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Background
The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules. The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition. Ten human homologs of TLRs (TLR1-10) have been described. Among this family of receptors, TLR2 and TLR4 have been most studied. These studies have suggested that TLR2 and TLR4 may serve as potential main mediators of LPS signaling. |
Antigen
A portion of amino acids 650-700 of human TLR4 was used as the immunogen for this antibody. |
Application Notes
The amino acid sequence used as immunogen for this antibodyis 100% homologous in human,rhesus monkey, macaque, chimpanzee, orangutan, gorilla, cow, bison, horse, opossum and boar, and 90% homologous in dog and rat. |
Genebank Info (Protein)
NP_612564.1 |
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Western blot analysis of TLR4 in human intestine lysate in the 1) absence and 2) presence of immunizing peptide using TLR4 antibody at 0.1 ug/ml. IMGENEX’s goat anti-rabbit Ig HRP secondary antibody (IMGENEX, 20301) and PicoTect ECL substrate solution (IMGENEX, 10087K) were used for this test. |
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Reference
1. Muzio, M. et al. J. Exp. Med. 187:2097-2101 (1998).
2. Medzhitov, R. et al. Cell. 91:295-298 (1997).
3. Chuang, TH. et al. Biochim. Biophys. Acta. 1518:157-161 (2001).
4. Takeuchi, O. et al. Immunity. 11:443 (1999).
5. Poltorak, A. et al. Proc. Natl. Acad. Sci USA. 97:2163-2167 (2000).
6. Medzhitov, R. et al. Nature. 388:394-397 (1997).
7. Jessica Nadigel, David Préfontaine, Carolyn J Baglole, François Maltais, Jean Bourbeau, David H Eidelman, Qutayba Hamid. Cigarette smoke increases TLR4 and TLR9 expression and induces cytokine production from CD8+ T cells in chronic obstructive pulmonary disease. Respir Res. 2011; 12(1): 149.
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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