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Polyclonal Antibody to eIF3G |
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| Catalog No: |
IMG-6310A |
| Contents: |
100 ug in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic. |
| Isotype: |
Rabbit IgG |
| Purification: |
Protein A Chromatography |
| Species: |
Chimpanzee, Human, Rhesus Monkey |
| Predicted React: |
Cow, Mouse, Opossum, Rat, Xenopus, Zebrafish |
| Host: |
Rabbit |
| Application: |
IHC (paraffin): 5 ug/ml
Western blot analysis: 4.0-5.0 ug/ml |
| Storage: |
Store at 4°C, stable for 6 months. For long-term storage, aliquot and store at -20°C. |
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Background
eIF3G is an RNA-associated protein involved in protein synthesis. It is a member of the eIF-3 subunit G family and the largest of the eukaryotic translation initiation factor family with eukaryotic translation initiation factor-3 (eIF3) being a multiprotein complex composed of at least 13 different subunits. eIF3G has a RRM (RNA recognition motif) domain and is known to interact with eIF3K. The function of eIF3G is to bind to the 40S ribosomal subunit and promote the binding of methionyl-tRNAi and mRNA. This subunit of eukaryotic translation initiation factor-3 also binds to the 18S rRNA. It is ubiquitously expressed in most tissues. |
Antigen
A portion of amino acids 50-100 of human eIF3G was used as the immunogen. |
Application Notes
The amino acid sequence used as immunogen is 100% homologous in human, rhesus monkey, chimpanzee, and dog, 94% homologous in mouse and cow, 88% homologous in rat, 83% homologous in opossum and zebrafish, and 72% homologous in Xenopus.
Peptide blocking: The response seen in western blot is reduced by over 50% in the presence of immunizing peptide. |
Genebank Info (Protein)
NP_003746.2 |
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Western blot analysis of eIF3G isoforms in 1) Hela cell lysate and 2) RAW cell lysate using IMG-6310A at 4.0 ug/ml. |
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Immunohistochemical analysis of eIF3G in formalin-fixed, paraffin-embedded human brain tissue using an isotype control (top left) and IMG-6310A (bottom left, right) at 5 ug/ml. |
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Reference
1. Bandyopadhyay, A. et al. Nucleic Acids Res. 27:1331-1337 (1999).
2. Block, KL. et al. J. Biol. Chem. 273:31901-31908 (1998).
3. Beausoleil, SA. et al. Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135 (2004).
4. Mayeur, GL. et al. Eur. J. Biochem. 270:4133-4139 (2003).
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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