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Peptide-affinity Purified Polyclonal Antibody to the ligand binding domain of TLR3 |
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| Catalog No: |
IMG-6315A |
| Contents: |
100 ug in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic. |
| Isotype: |
Rabbit Ig |
| Purification: |
Antigen Affinity Chromatography |
| Species: |
Chimpanzee, Cow, Gorilla, Human, Macaque, Mouse, Opossum, Rat |
| Predicted React: |
Dog, Cat |
| Host: |
Rabbit |
| Application: |
Flow (Intracellular): 1 ug/10^6 cells
IHC (paraffin): 5 ug/ml
Western blot analysis: 1-3 ug/ml |
| Storage: |
Store at 4°C, stable for 6 months. For long-term storage, aliquot and store at -20°C. |
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Background
The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules (1) and could constitute an important and unrecognized component of innate immunity in humans. The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition (2). Ten human homologs of TLRs (TLR1-10) have been described (3). TLR3 has a restricted expression pattern being expressed in dendritic cells (DC) and lymph nodes (4,5). The expression of TLR3 in a single cell type may indicate a specific role for this molecule in a restricted setting. TLR3 is activated by dsRNA, the genetic material of some viruses, and a viral replication intermediate in others (6). Crystal struture and mutational analyses of TLR3-ectodomain suggest that Histidine-539 and Asparagine-541 are important amino acid residues in the recognition of dsRNA. H539E and N541A mutations abolish the dsRNA binding and ligand-dependent activation of TLR3 (7). This polyclonal antibody may block dsRNA binding to TLR3. |
Antigen
A peptide containing H-539 and N-541, two important amino acids involved in the binding of dsRNA to TLR3, was used as the immunogen. |
Application Notes
The amino acid sequence used as immunogen is 100% homologous in human, rhesus monkey, macaque, orangutan, gorilla, chimpanzee, cow, and opossum, 95% homologous in dog and wild boar, 90% homologous in cat and rat, and 85% homologous in mouse. |
Genebank Info (Protein)
AAH59372 |
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Western blot analysis of TLR3 in rat, mouse and human tissues and cells using TLR3 antibody at 1-3 ug/ml. |
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Flow cytometric analysis of TLR3 in RAW cells using TLR3 antibody (red) and isotype control antibody (green) at 1 ug/10^6 cells. |
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Immunohistochemical analysis of TLR3 in formalin-fixed, paraffin-embedded human stomach tumor using an isotype control antibody (top) and TLR3 antibody (bottom) at 5 ug/ml. |
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Reference
1. Muzio, M. et al. J Immunol. 164:5998-6004 (2000).
2. Muzio, M. et al. J. Exp. Med. 187:2097-2101 (1998).
3. Medzhitov, R. et al. Cell. 91: 295-298 (1997).
4. Rock. FL. et al. Proc. Natl. Acad. Sci. U.S.A. 95 (2):588-593 (1998).
5. Muzio, M. et al. J Leukoc Biol. 67:450-456 (2000).
6. Alexopoulou, L. et al. Nature 413, 732–738 (2001).
7. Bell, JK et al. Proc. Natl. Acad. Sci. U.S.A. 103 (23): 8792–8797 (2006).
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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