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Peptide-affinity Purified Polyclonal Antibody to eIF3D |
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| Catalog No: |
IMG-6316A |
| Contents: |
100 ug in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic. |
| Isotype: |
Rabbit Ig |
| Purification: |
Antigen Affinity Chromatography |
| Species: |
Chimpanzee, Human, Macaque, Mouse, Orangutan, Rat, Rhesus Monkey, Zebrafish |
| Predicted React: |
Chicken, Cow, Dog, Horse, Opossum |
| Host: |
Rabbit |
| Application: |
Western blot analysis:0.005-0.01 ug/ml |
| Storage: |
Store at 4°C, stable for 6 months. For long-term storage, aliquot and store at -20°C. |
Recommended
Positive Control: |
Brain |
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Background
eIF3D is a protein belonging to the largest of the eukaryotic translation initiation factor family, eukaryotic translation initiation factor-3 (eIF3). eIF3 is a multiprotein complex composed of at least 13 different subunits. eIF3D binds to the 40S ribosomal subunit and thus helps in maintaining the 40S and 60S ribosomal subunits in a dissociated state. An important role of eIF3D is the formation of the 40S initiation complex by interacting with the ternary complex of eIF2/GTP/methionyl-tRNA, and promotes mRNA binding. eIF3D is the major RNA binding subunit of the eIF3 complex. It associates with the subunit p170 of eIF-3. Ubiquitous expression of eIF3D is seen in most of the tissues and it is phosphorylated upon DNA damage, likely by ATM or ATR. |
Antigen
A portion of amino acids 500-550 of human eIF3D was used as the immunogen. |
Application Notes
The amino acid sequence used as immunogen is 100% homologous in human, rhesus monkey, chimpanzee, orangutan, macaque and zebrafish, 94% homologous in cow, 90% homologous in opossum and 88% homologous in chicken, horse, platypus, rat, mouse and dog. |
Genebank Info (Protein)
NP_003744.1 |
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Western blot analysis of eIF3D in human brain lysate in the 1) absence and 2) presence of immunizing peptide, 3) mouse brain and 4) rat brain lysate using IMG-6316A at 0.01 ug/ml, 0.01 ug/ml, 0.005 ug/ml and 0.01 ug/ml, respectively. |
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Reference
1. Asano, K. et al. J. Biol. Chem. 272:27042-27052 (1997).
2. Rush, J. et al. Nat. Biotechnol. 23:94-101 (2005).
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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