|
|
|
| |
Peptide-affinity Purified Polyclonal Antibody to ARRB2 |
|
|
| |
|
|
| |
|
| Catalog No: |
IMG-6325A |
| Contents: |
100 ug in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic. |
| Isotype: |
Rabbit Ig |
| Purification: |
Antigen Affinity Chromatography |
| Species: |
Cow, Dog, Horse, Human, New World monkey, Mouse, Rat |
| Predicted React: |
Pufferfish, Xenopus, Zebrafish |
| Host: |
Rabbit |
| Application: |
IHC (paraffin): 5 ug/ml
Western blot analysis: 0.25-0.5 ug/ml |
| Storage: |
Store at 4°C, stable for 6 months. For long-term storage, aliquot and store at -20°C. |
Recommended
Positive Control: |
HUVEC cell lysate |
|
 |
|
|
|
|
|
|
Background
Beta-arrestin-2, or ARRB2, is an adaptor protein belonging to the arrestin family with an N- arrestin domain and a C- arrestin domain. ARRB2 is a regulatory protein involved in heterotrimeric G protein-coupled receptor desensitization and is known to regulate beta2-adrenergic receptor (beta2AR) function by enhancing beta2AR mediated nuclear translocation of ERK. ARRB2 binds to phosphorylated beta2ARs, thereby causing a significant impairment of their capacity to activate G(S) proteins. Along with AIP4, ARRB2 acts as an endosomal sorting molecule that mediates CXCR4 entry into a degradative pathway. It may also be involved in hormone-specific desensitization of TSH receptors. ARRB2 is a Ca2+ binding protein of the retinal outer segments and binds to P-rhodopsin and is also involved in synaptic transmission in photoreceptor cells. It is widely expressed in most of the tissues, especially neuronal tissues and spleen. |
Antigen
A peptide sequence corresponding to amino acids 23-40 (GKRDFVDHLDKVDPVDG) of human ARRB2 was used as the immunogen, Gen Bank no. gb|ABG47460.1|. |
Application Notes
The amino acid sequence used as immunogen is 100% homologous in human, rhesus monkey, chimpanzee, mouse, rat, dog, horse and cow, and 94% homologous in pufferfish, zebrafish and xenopus. |
Genebank Info (Protein)
P32121 |
| |
|
Western blot analysis of ARRB2 in HUVEC cell lysate in the 1) absence and 2) presence of immunizing peptide using IMG-6325A at 0.25 ug/ml. IMGENEX’s goat anti-rabbit Ig HRP secondary antibody (IMGENEX, 20301) and PicoTect ECL substrate solution (IMGENEX, 10087K) were used for this test. |
|
|
|
|
Immunohistochemical analysis of ARRB2 in formalin-fixed, paraffin-embedded human stomach tumor tissue using an isotype control (top left) and IMG-6325A (bottom left, right) at 5 ug/ml. |
|
|
|
|
|
| - |
 |
|
Reference
1. Alloway, PG. et al. Neuron. 28:129-138 (2000).
2. Attramadal, H. et al. J. Biol. Chem. 267:17882-17890 (1992).
3. Chen, W. et al. Science. 306:2257-2260 (2004).
4. Chen, W. et al. Science. 301:1394-1397 (2003).
|
|
|
|
Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
|
|
|
|
|
