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Product : Antibody >> Antigen Affinity Purified , Cytokine & Chemokine , IHCProTM , Immunohistochemistry , Innate Immunology >> EBI3
      
 

Peptide-affinity Purified Polyclonal Antibody to EBI3 

 
Cat. No.DescriptionFormatUnitPrice(USD)QuantityBulk Quotation
IMG-6327A
EBI3Purified, Peptide Affinity
0.1 mg
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Catalog No: IMG-6327A
Imgenex is now a part of Novus Biologicals! All orders, billing and shipments will be received from Novus. Please contact Novus directly for any questions – novus@novusbio.com. To insure proper processing of you order, please insure that your purchase orders show Novus Biologicals as the vendor.
Contents: 100 ug in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Isotype: Rabbit Ig
Purification: Antigen Affinity Chromatography
Species: Human, Mouse, Rat
Host: Rabbit
Application: IHC (paraffin): 5 ug/ml
Western blot analysis: 0.5-2.0 ug/ml
Storage: Store at 4°C, stable for 6 months. For long-term storage, aliquot and store at -20°C.
 

Background
EBI3 is a 228 amino acid containing pro- and anti-inflammatory cytokine belonging to the type I cytokine receptor family and contains two fibronectin type-III domain. It forms a heterodimer with IL-27 and is also a component of the IL-12 heterodimer. A protein involved in regulated cell-mediated immune responses, EBI3 has an important role in regulating T helper cell development, suppressing T-cell proliferation, stimulating cytotoxic T-cell activity, inducing isotype switching in B-cells, and has diverse effects on innate immune cells. Target cells of EBI3 include CD4 T helper cells, type 1 effector cells (TH1), type 2 effector cells (TH2) and IL-17 producing helper T-cells (TH17). It drives rapid clonal expansion of naive but not memory CD4 T-cells. EBI3 is important in anti-tumor as well as anti-angiogenic activity with activation and production of anti-angiogenic chemokines. It also strongly synergizes with IL-12 to trigger interferon-gamma/IFN-gamma production of naive CD4 T-cells.

Antigen
A portion of amino acids 200-230 of mouse EBI3 was used as the immunogen.

Application Notes
The amino acid sequence used as immunogen is 100% homologous in mouse (isoforms CRA_a and CRA_b) and rat and 72% homologous in human, dog and rhesus monkey.

View additional EBI3 antibodies              

Genebank Info (Protein): NP_056581.1
Gene ID: 50498
 
EBI3 Antibody WB

Western blot analysis of EBI3 in RAW cell lysate in the 1) absence and 2) presence of immunizing peptide using IMG-6327A at 0.5 ug/ml.  IMGENEX’s goat anti-rabbit Ig HRP secondary antibody (IMGENEX, 20301) and PicoTect ECL substrate solution (IMGENEX, 10087K) were used for this test.

EBI3 Antibody IHC

Formalin-fixed, paraffin-embedded mouse brain tissue stained with EBI3 antibody at 5 ug/ml.

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Reference
1. Batten, M. et al. J. Mol. Med. 85:661-672 (2007).


Research purposes only. Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.


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