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Peptide-affinity Purified Polyclonal Antibody to EBI3 |
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| Catalog No: |
IMG-6327A |
| Contents: |
100 ug in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic. |
| Isotype: |
Rabbit Ig |
| Purification: |
Antigen Affinity Chromatography |
| Species: |
Human, Mouse, Rat |
| Host: |
Rabbit |
| Application: |
IHC (paraffin): 5 ug/ml
Western blot analysis: 0.5-2.0 ug/ml |
| Storage: |
Store at 4°C, stable for 6 months. For long-term storage, aliquot and store at -20°C. |
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Background
EBI3 is a 228 amino acid containing pro- and anti-inflammatory cytokine belonging to the type I cytokine receptor family and contains two fibronectin type-III domain. It forms a heterodimer with IL-27 and is also a component of the IL-12 heterodimer. A protein involved in regulated cell-mediated immune responses, EBI3 has an important role in regulating T helper cell development, suppressing T-cell proliferation, stimulating cytotoxic T-cell activity, inducing isotype switching in B-cells, and has diverse effects on innate immune cells. Target cells of EBI3 include CD4 T helper cells, type 1 effector cells (TH1), type 2 effector cells (TH2) and IL-17 producing helper T-cells (TH17). It drives rapid clonal expansion of naive but not memory CD4 T-cells. EBI3 is important in anti-tumor as well as anti-angiogenic activity with activation and production of anti-angiogenic chemokines. It also strongly synergizes with IL-12 to trigger interferon-gamma/IFN-gamma production of naive CD4 T-cells. |
Antigen
A portion of amino acids 200-230 of mouse EBI3 was used as the immunogen. |
Application Notes
The amino acid sequence used as immunogen is 100% homologous in mouse (isoforms CRA_a and CRA_b) and rat and 72% homologous in human, dog and rhesus monkey. View additional EBI3 antibodies |
Genebank Info (Protein)
NP_056581.1 |
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Western blot analysis of EBI3 in RAW cell lysate in the 1) absence and 2) presence of immunizing peptide using IMG-6327A at 0.5 ug/ml. IMGENEX’s goat anti-rabbit Ig HRP secondary antibody (IMGENEX, 20301) and PicoTect ECL substrate solution (IMGENEX, 10087K) were used for this test. |
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Formalin-fixed, paraffin-embedded mouse brain tissue stained with EBI3 antibody at 5 ug/ml. |
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Reference
1. Batten, M. et al. J. Mol. Med. 85:661-672 (2007).
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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