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Monoclonal Antibody to CD14 (Clone RPA-M1) PerCP-Cy5.5 Conjugate |
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| Catalog No: |
IMG-6350H |
| Contents: |
100 tests in 1 ml PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic. |
| Isotype: |
Mouse IgG1, Kappa |
| Clone: |
RPA-M1 |
| Purification: |
Protein G Chromatography |
| Species: |
Human |
| Host: |
Mouse |
| Application: |
Flow (Cell Surface): 10 ul/10^6 cells (0.05 ug)
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| Storage: |
Store at 4°C, stable for 6 months. DO NOT FREEZE; MATERIAL IS LIGHT-SENSITIVE. |
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Background
CD14 a well known cell-activating receptor for lipopolysaccharide-binding protein and peptidoglycan is an important regulator of lipopolysaccharide (LPS)-dependent signal transduction and is a part of the multi-protein complex containing at least CD14, MD-2 and TLR4. Encoded by a single-copy gene, CD14 contains 11 LRR (leucine-rich) repeats and exists in two forms a glycosylphosphatidylinositol-anchored membrane protein (mCD14) and a monocyte or liver-derived soluble serum protein (sCD14) that lacks the anchor. The soluble form of CD14 functions in LPS sensitivity to cells lacking the membrane form and its increased expression is reported to be associated with inflammatory infectious diseases and high mortality in gram-negative shock. CD14 an important component of the innate immune response preferentially expressed on mature cells of the monocytic lineage mediates the bacterial lipopolysaccharide (LPS) signaling, thus leading to NF-kappa-B activation, cytokine secretion and inflammatory response. Moreover it is also known to be involved in the clearance of apoptotic cells, up-regulation of cell surface molecules (adhesion molecules) and also in regulating IgE levels. |
Antigen
Human PHA-stimulated PBMC were used as immunogen. |
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Cell surface flow cytometric analysis of CD14 in 10^6 human monocytes using 10 ul of IMG-6350H. Green represents PerCP-Cy5.5 conjugated isotype control (IMGENEX, 20109H); red represents anti-CD14 antibody. IMGENEX's cell surface flow kit (10084K) was used for this test. |
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Reference
1. Sandanger, O. etal. J Immunol. 182:588-595 (2009).
2. Kurt-Jones, EA. et al. Nature Immun. 1:398-401 (2000).
3. Devitt, A. et al. Nature 392:505-509 (1998).
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for six months from the date of purchase.
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