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Polyclonal Antibody to BMI1 |
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| Catalog No: |
IMG-6362A |
| Contents: |
100 ug in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic. |
| Isotype: |
Rabbit IgG |
| Purification: |
Protein A Chromatography |
| Species: |
Chimpanzee, Human, Macaque, Mouse, Rat |
| Predicted React: |
Cat, Cow, Dog, Horse, Opossum |
| Host: |
Rabbit |
| Application: |
IHC (paraffin): 5 ug/ml
Western blot analysis: 2.0-3.0 ug/ml |
| Storage: |
Store at 4°C, stable for 6 months. For long-term storage, aliquot and store at -20°C. |
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Background
BMI1 is a 326 amino acid containing protooncogene belonging to the polycomb gene family with a RING-type zinc finger domain. A transcription factor and a senescence regulator, BMI1 is a component of the Polycomb group (PcG) multiprotein PRC1 complex, required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. An important role of BMI1 has been reported in regulating proliferation of stem cells and tumor cells in diverse adult epithelial tissues involved in human colorectal carcinogenesis by repressing the INK4a/ARF proteins (CDKN2A). This protein also plays an essential role in the generation of self-renewing adult hematopoietic stem cells and in the maintenance and expansion of immature granule cell precursors. BMI1 cooperates with the MYC oncogene to produce B-lymphomas. Ubiquitous expression is seen in most of the tissues. |
Antigen
A portion of amino acids 270-320 of human BMI1 was used as the immunogen. |
Application Notes
The amino acid sequence used as immunogen is 100% homologous in human, chimpanzee, rat, and macaque, 94% homologous in horse, cow, dog, cat, mouse and 88% homologous in opossum. |
Genebank Info (Protein)
NP_005171.4 |
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Western blot analysis of BMI1 in skeletal muscle lysate. Lane 1 shows pre-immune sera. Lanes 2, 3 and 4 show IMG-6362A tested on human, mouse and rat muscle lysate at 2 ug/ml, respectively. IMGENEX’s goat anti-rabbit Ig HRP secondary antibody (IMGENEX, 20301) and PicoTect ECL substrate solution (IMGENEX, 10087K) were used for this test. |
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Immunohistochemical analysis of BMI1 in formalin-fixed, paraffin-embedded human cervix tissue using an isotype control (top left) and IMG-6362A (bottom left, right) at 5 ug/ml. |
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Reference
1. Wang, H. et al. Nature. 431:873-878 (2004).
2. Satijn, DPE. et al. Molec. Cell. Biol. 17:4105-4113 (1997).
3. Park, I. et al. Nature. 423:302-305 (2003).
4. Sangiorgi, E. et al. Nature Genet. 40:915-920 (2008).
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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