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Product : Antibody >> Apoptosis , Innate Immunology , New Antibodies , Stem Cell Markers , Transcription Factors >> BMI1
      
 

Polyclonal Antibody to BMI1

 
Cat. No.DescriptionFormatUnitPrice(USD)QuantityBulk Quotation
IMG-6362A
BMI1Purified
0.1 mg
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Catalog No: IMG-6362A
Imgenex is now a part of Novus Biologicals! All orders, billing and shipments will be received from Novus. Please contact Novus directly for any questions – novus@novusbio.com. To insure proper processing of you order, please insure that your purchase orders show Novus Biologicals as the vendor.
Contents: 100 ug in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Isotype: Rabbit IgG
Purification: Protein A Chromatography
Species: Chimpanzee, Human, Macaque, Mouse, Rat
Predicted React: Horse, Dog, Cow, Cat, Opossum
Host: Rabbit
Application: IF/ICC: 5 ug/ml
IHC (paraffin): 5 ug/ml
Western blot analysis: 2.0-3.0 ug/ml
Storage: Store at 4°C, stable for 6 months. For long-term storage, aliquot and store at -20°C.
 

Background
BMI1 is a 326 amino acid containing protooncogene belonging to the polycomb gene family with a RING-type zinc finger domain. A transcription factor and a senescence regulator, BMI1 is a component of the Polycomb group (PcG) multiprotein PRC1 complex, required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. An important role of BMI1 has been reported in regulating proliferation of stem cells and tumor cells in diverse adult epithelial tissues involved in human colorectal carcinogenesis by repressing the INK4a/ARF proteins (CDKN2A). This protein also plays an essential role in the generation of self-renewing adult hematopoietic stem cells and in the maintenance and expansion of immature granule cell precursors. BMI1 cooperates with the MYC oncogene to produce B-lymphomas. Ubiquitous expression is seen in most of the tissues. 

Antigen
A portion of amino acids 270-320 of human BMI1 was used as the immunogen.

Application Notes
The amino acid sequence used as immunogen to generate the BMI1 antibody is 100% homologous in human, chimpanzee, rat, and macaque, 94% homologous in horse, cow, dog, cat, mouse and 88% homologous in opossum.

Genebank Info (Protein): NP_005171.4
Gene ID: 648
SwissProt: P35226
 
BMI1 Antibody

Western blot analysis of BMI1 in skeletal muscle lysate.  Lane 1 shows pre-immune sera.  Lanes 2, 3 and 4 show IMG-6362A tested on human, mouse and rat muscle lysate at 2 ug/ml, respectively.  IMGENEX’s goat anti-rabbit Ig HRP secondary antibody (IMGENEX, 20301) and PicoTect ECL substrate solution (IMGENEX, 10087K) were used for this test.

BMI1 Antibody IMG-6362A

Immunofluorescence of BMI1 in human oral squamous cell carcinoma (UPCI:SCC029B) cells. These cells are part of a large collection of HNSCC (head and neck SCC) lines established by the Gollin lab (White et al., 2007). The BMI1 antibody was used at 5 ug/ml. Staining is primarily nuclear, some cytoplasmic staining is also observed. Data courtesy of Hatem Kaseb, MD and Susanne M. Gollin, PhD.

BMI1

Immunohistochemical analysis of BMI1 in formalin-fixed, paraffin-embedded human cervix tissue using an isotype control (top left) and IMG-6362A (bottom left, right) at 5 ug/ml.

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Reference
1. Wang, H. et al. Nature. 431:873-878 (2004).
2. Satijn, DPE. et al. Molec. Cell. Biol. 17:4105-4113 (1997).
3. Park, I. et al. Nature. 423:302-305 (2003).
4. Sangiorgi, E. et al. Nature Genet. 40:915-920 (2008).
5. White JS, JL Weissfeld, CCR Ragin, KM Rossie, CL Martin, M Shuster, CS Ishwad, JC Law, EN Myers, JT Johnson, SM Gollin. The influence of clinical and domographic fisk factors on the establishment of head and neck squamous cell carcinoma cell lines. Oral Oncol 43:701-712 (2007).


Research purposes only. Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.


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