|
|
|
| |
Polyclonal Antibody to Vigilin/HDLBP |
|
|
| |
|
|
| |
|
| Catalog No: |
IMG-6365A |
| Contents: |
100 ug in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic. |
| Isotype: |
Rabbit IgG |
| Purification: |
Protein G Chromatography |
| Species: |
Chimpanzee, Dog, Horse, Human, Mouse, Pig, Rat |
| Host: |
Rabbit |
| Application: |
Western blot analysis: 3-5 ug/ml |
| Storage: |
Store at 4°C, stable for 6 months. For long-term storage, aliquot and store at -20°C. |
|
 |
|
|
|
|
|
|
Background
Vigilin, also known as high density lipoprotein binding protein (HDLBP), is a 1268 amino acid containing cytoplasmic protein in close association with the endoplasmic reticulum with fourteen K homology (KH). A transport protein, widely expressed in most of tissues, appears to play a role in cell sterol metabolism. It specifically binds to HDL (high density lipoprotein) molecules and may function to protect cells from over-accumulation of cholesterol by removal of excess cellular cholesterol. |
Antigen
A portion of amino acids 500-550 and 900-950 of human Vigilin was used as the immunogen. |
Application Notes
The amino acid sequence used as immunogen is 100% homologous in human, mouse, rat, chimp, cat, dog and horse. |
| Genebank Info (Protein): NP_005327 |
| Gene ID: 3069 |
| SwissProt: Q00341 |
| |
|
Western blot analysis of Vigilin in A) Jurkat cell lysate and B) RAW cell lysate using IMG-6365A at 3 and 5 ug/ml, respectively. IMGENEX’s goat anti-rabbit Ig HRP secondary antibody (IMGENEX, 20301) and PicoTect ECL substrate solution (IMGENEX, 10087K) were used for this test. |
|
|
|
|
|
| - |
 |
|
Reference
1. Plenz, G. et al. Hum. Genet. 93:575-582 (1994).
2. LeBoeuf, RC. et al. Genomics. 23:296-298 (1994).
3. Kugler, S. et al. Europ. J. Biochem. 238:410-417 (1996).
|
|
|
|
Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
|
|
|
|
|
