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Polyclonal Antibody to NALP10 |
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| Catalog No: |
IMG-6366A |
| Contents: |
100 ug in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic. |
| Isotype: |
Rabbit IgG |
| Purification: |
Protein A Chromatography |
| Species: |
Human |
| Predicted React: |
Horse, Rhesus Monkey |
| Host: |
Rabbit |
| Application: |
Western blot analysis: 1.0-2.0 ug/ml |
| Storage: |
Store at 4°C, stable for 6 months. For long-term storage, aliquot and store at -20°C. |
Recommended
Positive Control: |
HeLa and RAW cell lysates |
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Background
NALP10, a member of the NALP protein family, is reported to have a regulatory role in the innate immune system and forms a large signal-induced multiprotein complex, the inflammasome, which activates the pro-inflammatory caspases, caspase-1 and caspase-5. Unlike other members of the family NALP10 lacks the LRR (leucine rich repeat) region but contains a DAPIN domain and a NACHT domain. Immunoprecipitation reports suggest that NALP10 interacted with ASC, CASP1, and IL-1B and inhibits NF-kB activation, CASP1/caspase-1-dependent IL-1B secretion and apoptosis induced by ASC (PYCARD) in a dose-dependent manner. Expression of NALP10 is enhanced by stimulation with lipopolysaccharide. Experiments with NALP10 suggest that it acts as a multifunctional negative regulator of inflammation and apoptosis. Wide expression of NALP10 is reported with highest levels in heart, brain and skeletal muscle. |
Antigen
A portion of amino acids 493-509 of human NALP10 REVQRLLEVKEQEGNDE protein was used as the immunogen. |
Application Notes
The amino acid sequence used as immunogen is 100% homologous in human, 94% homologous in rhesus monkey and horse and 83% homologous in Tetradon.
The theoretical size of NALP10 is approximately 71 kD. IMG-6366A detects a protein at approximately 60 kD and the response is completely blocked by the immunizing peptide. The reason for the size discrepency is not known. |
Genebank Info (Protein)
NP_789791.1 |
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Western blot analysis of NALP10 in HeLa cell lysate in the 1) absence and 2) presence of immunizing peptide and 3) RAW cell lysate using IMG-6366A at 1.0 ug/ml. IMGENEX’s goat anti-rabbit Ig HRP secondary antibody (IMGENEX, 20301) and PicoTect ECL substrate solution (IMGENEX, 10087K) were used for this test. |
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Reference
1. Tschopp, J. et al. Nature Rev. Molec. Cell Biol. 4:95-104 (2003).
2. Wang, Y. et al. Int. Immun. 16:777-786 (2004).
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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