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Peptide-affinity Purified Polyclonal Antibody to AGAP2 |
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| Catalog No: |
IMG-6377A |
| Contents: |
100 ug in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic. |
| Isotype: |
Rabbit Ig |
| Purification: |
Antigen Affinity Chromatography |
| Species: |
Human, New World monkey |
| Predicted React: |
Horse |
| Host: |
Rabbit |
| Application: |
IHC (paraffin): 5 ug/ml
Western blot analysis: 2.0-3.0 ug/ml
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| Storage: |
Store at 4°C, stable for 6 months. For long-term storage, aliquot and store at -20°C. |
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Background
AGAP2 (also known as PIKE-A), a putative oncogene, shows elevated expression in cancer cells compared to non-transformed cells and has been implicated as regulators of cancer cell invasion into normal tissues. A contributor to brain, eye, breast and liver cancers, AGAP2 belongs to the centaurin gamma-like family with two ANK repeats, an Arf-GAP domain, a Miro domain and a PH domain. It is a GTPase-activating protein (GAP) for ARF1 and ARF5, which also shows strong GTPase activity. The Arf-GAP domain interacts with G domain and may regulate its GTPase activity. GAP activity of AGAP2 is stimulated by phosphatidylinositol 4,5-bisphosphate (PIP2) and, to a lesser extent, by phosphatidylinositol 3,4,5-trisphosphate (PIP3). Phosphatidic acid potentiates PIP2 stimulation. Several isoforms of AGAP2 have been reported. |
Antigen
A portion of amino acids 20-60 of human AGAP2 was used as the immunogen. |
Application Notes
The amino acid sequence used as immunogen is 100% homologous in human and monkey and 83% homologous in horse. |
| Genebank Info (Protein): NP_055585 |
| Gene ID: 116986 |
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Western blot analysis of AGAP2 in Jurkat cell lysate in the 1) absence and 2) presence of immunizing peptide using IMG-6377A at 2.0 ug/ml. IMGENEX’s goat anti-rabbit Ig HRP secondary antibody (IMGENEX, 20301) and PicoTect ECL substrate solution (IMGENEX, 10087K) were used for this test. |
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Immunohistochemical analysis of AGAP2 in formalin-fixed, paraffin-embedded human brain tissue using an isotype control (top left) and IMG-6377A (bottom left, right) at 5 ug/ml. |
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Reference
1. Ahn, J.-Y. et al. Proc. Nat. Acad. Sci. 101:6993-6998 (2004).
2. Liu, Z. et al. Molec. Cell. 29:665-678 (2008).
3. Ye, K. et al. Nature. 415:541-544 (2002).
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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