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Product : Antibody >> Chromatin , Development & Differe.. , Inflammation , New Antibodies , Tumor BioMarkers >> ARAP2
      
 

Polyclonal Antibody to ARAP2

 
Cat. No.DescriptionFormatUnitPrice(USD)QuantityBulk Quotation
IMG-6381A
ARAP2Purified
0.1 mg
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Catalog No: IMG-6381A
Imgenex is now a part of Novus Biologicals! All orders, billing and shipments will be received from Novus. Please contact Novus directly for any questions – novus@novusbio.com. To insure proper processing of you order, please insure that your purchase orders show Novus Biologicals as the vendor.
Contents: 100 ug in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Isotype: Rabbit IgG
Purification: Protein A Chromatography
Species: Human
Predicted React: Chimpanzee, Cow, Horse, New World monkey
Host: Rabbit
Application: IHC (paraffin): 5 ug/ml
Western blot analysis: 1.0-2.0 ug/ml
Storage: Store at 4°C, stable for 6 months. For long-term storage, aliquot and store at -20°C.
Recommended
Positive Control:
Human kidney lysate
 

Background
ARAP2 also known as Centaurin-delta-1 is a protein with ARF-GAP, RHO-GAP, ankyrin repeat, RAS-associating, and pleckstrin homology domains. A phosphatidylinositol-3, 4, 5-trisphosphate-dependent GTPase-activating protein, ARAP2, binds to RhoA-GTP, but lacks the predicted catalytic arginine in the RHO-GAP domain and does not have RHO-GAP activity. It modulates actin cytoskeleton remodeling by regulating ARF and RHO family members. Usually ARAP2 is activated by phosphatidylinositol-3,4,5-trisphosphate (PtdIns(3,4,5)P3) binding and can be activated by phosphatidylinositol-3,4-bisphosphate (PtdIns(3,4,5)P2) binding, albeit with lower efficiency. ARAP2 associates with focal adhesions and functions downstream of RhoA to regulate focal adhesion dynamics. It is usually detected in brain, thymus, lymph node, thyroid, spinal cord, trachea, heart, skeletal muscle, spleen, kidney, liver, placenta, lung and peripheral blood leukocytes. 

Antigen
A portion of amino acids 1670-1720 of human ARAP2 was used as the immunogen.

Application Notes
The amino acid sequence used as immunogen is 100% homologous in human (isoforms CRA_a, CRA_b, and CRA_c), 93% homologous in monkey, 87% homologous in chimpanzee and 81% homologous in cow and horse.

Genebank Info (Protein): NP_056045
Gene ID: 116984
 
ARAP2 Antibody

Western blot analysis of ARAP2 in human kidney lysate in the 1) absence and 2) presence of immunizing peptide using IMG-6381A at 1.0 ug/ml.  IMGENEX’s goat anti-rabbit Ig HRP secondary antibody (IMGENEX, 20301) and PicoTect ECL substrate solution (IMGENEX, 10087K) were used for this test.

ARAP2 Antibody IMG-6381A

Immunohistochemical analysis of ARAP2 in formalin-fixed, paraffin-embedded human brain tissue using an isotype control (top left) and IMG-6381A (bottom left, right) at 5 ug/ml.

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Reference
1. Nagase, T. et al. DNA Res. 5:31-39 (1998).
2. Miura, K. et al. Molec. Cell. 9:109-119 (2002).


Research purposes only. Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.


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