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Polyclonal Antibody to PLD2 |
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| Catalog No: |
IMG-6404A |
| Contents: |
100 ul of crude rabbit sera with 50% glycerol and 0.025% sodium azide. Sodium azide is highly toxic. |
| Isotype: |
Rabbit Ig |
| Purification: |
Crude rabbit sera |
| Species: |
Chimpanzee, Human |
| Predicted React: |
Cow, Dog, Horse, Mouse, Opossum, Pufferfish, Rhesus Monkey |
| Host: |
Rabbit |
| Application: |
IHC (paraffin): 1:50 |
| Storage: |
Store at -20°C. |
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Background
PLD2 is a peripheral membrane protein belonging to phospholipase D family with a PH domain, two PLD phosphodiesterase domains and a PX (phox homology) domain that plays a regulatory rather than catalytic role. PLD2 catalyzes the hydrolysis of phosphatidylcholine (PC) to produce phosphatidic acid and choline. PLD2 may function in regulated secretion, signal-induced cytoskeletal regulation and/or endocytosis, transcriptional regulation, and cell cycle control. It is usually stimulated by phosphatidylinositol 4,5-bisphosphate (PIP2) and activated by the ADP-ribosylation factor-1 (ARF-1). PLD2 is activated by agonist stimulation of both tyrosine kinase and G protein-coupled receptors and is also known to interact with EGFR and PIP5K1A. PLD2, localized to plasma membrane caveolae, is ubiquitously expressed in most tissues. |
Antigen
Amino acids 199-216 DLGRKGLEGMIRKRSGGH of human PLD2 protein were used as the immunogen. |
Application Notes
The amino acid sequence used as immunogen is 100% homologous in human (isoforms CRA_b and CRA_c) and chimpanzee, 88% homologous in mouse, rat, horse, rhesus monkey, cow, opossum and dog, and 83% homologous in zebrafish, pufferfish and Xenopus. |
| Genebank Info (Protein): NP_002654 |
| Gene ID: 5338 |
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Immunohistochemical analysis of PLD2 in formalin-fixed, paraffin-embedded human stomach tissue using preimmune sera (top) and IMG-6404A (bottom) at 1:50. |
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Reference
1. Liscovitch, M. et al. Biochem. J. 345:401-415 (2000).
2. Lopez, I. et al. J. Biol. Chem. 273:12846-12852 (1998).
3. Park, SH. et al. Cytogenet. Cell Genet. 82:225 (1998).
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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