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HSP27 Phospho Polyclonal Antibody (Ser78)| Peptide-Affinity Purified |
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| Catalog No: |
IMG-6425A |
| Contents: |
20 ug phospho antibody in 100 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic. |
| Isotype: |
Rabbit Ig |
| Purification: |
Antigen Affinity Chromatography |
| Species: |
Chimpanzee, Dog, Horse, Human, Macaque |
| Host: |
Rabbit |
| Application: |
Western blot analysis: use phospho antibody at 0.01-0.1 ug/ml |
| Storage: |
Store phospho antibody at 4°C, stable for 6 months. For long-term storage, aliquot and store phospho antibody at -20°C. |
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Background
HSP27, also known as Heat shock 27 kDa protein, is a cytoplasmic protein in interphase cells and colocalizes with mitotic spindles in mitotic cells. It translocates to the nucleus during heat shock. HSP27 belongs to the small heat shock protein (HSP20) family and is known to be an important protein in stress resistance and actin organization and associates with alpha- and beta-tubulin, microtubules and CRYAB. It also interacts with other heat shock proteins like HSPB8 and HSPBAP1. HSP27 is expressed in response to environmental stresses such as heat shock, or estrogen stimulation in MCF-7 cells. Ubiquitous expression is seen in most tissues with highest levels in heart and in tissues composed of striated and smooth muscle. Defects in HSPB1 are the cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuronopathy type 2B (HMN2B). |
Antigen
A portion of human HSP27 protein containing a phosphorylated serine residue at position 78 was used as the immunogen. |
Application Notes
The amino acid sequence used as immunogen is 100% homologous in human, horse, macaque, chimpanzee and dog, 90% homologous in opossum, cow, zebrafish, boar and Xenopus and 81% homologous in rat and mouse. |
Genebank Info (Protein)
P04792 |
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Western blot analysis of HSP27 (Ser78) in recombinant fusion protein containing 1) a phosphorylated serine at position 78 and 2) an unphosphorylated serine at position 78, using HSP27 phospho antibody at 0.01 ug/ml. IMGENEX’s goat anti-rabbit Ig HRP secondary antibody (IMGENEX, 20301) and PicoTect ECL substrate solution (IMGENEX, 10087K) were used for this test. |
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Reference
1. Houlden, H. et al. Neurology. 71:1660-1668 (2008).
2. Rayner, K. et al. Circ. Res. 103:133-141 (2008).
3. Stock, AD. et al. Am. J. Med. Genet. 120A:320-325 (2003).
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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