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Polyclonal Antibody to Beta-arrestin 1 |
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| Catalog No: |
IMG-6428A |
| Contents: |
100 ug in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic. |
| Isotype: |
Rabbit IgG |
| Purification: |
Protein A Chromatography |
| Species: |
Bovine, Human, Mouse, Rat, Zebrafish |
| Predicted React: |
Chicken, Frog |
| Host: |
Rabbit |
| Application: |
Western blot analysis: 2.0-3.0 ug/ml |
| Storage: |
Store at 4°C, stable for 6 months. For long-term storage, aliquot and store at -20°C. |
Recommended
Positive Control: |
Human Testis lysate |
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Background
Beta-arrestin 1 is a 418 amino acid containing signaling neurotransmitter that belongs to the arrestin family with an arrestin N terminal and an arrestin C terminal domain. A regulator of beta-adrenergic receptor function, Beta-arrestin 1 seems to bind to phosphorylated beta-adrenergic receptors, thereby causing a significant impairment of their capacity to activate G(S) proteins. It is also reported to act as an activator of the lymphoid enhancer factor (LEF) transcription factor. Beta-arrestin 1 plays a crucial role in ubiquitination and down-regulation of the insulin-like growth factor-1 receptor by acting as an adaptor for the MDM2 E3 ligase. Purified beta-arrestin inhibits the signaling function of BARK-phosphorylated beta-adrenergic receptors by more than 75%, but not that of rhodopsin, and is also involved in synaptic transmission in photoreceptor cells. It is widely expressed in most tissues. |
Antigen
A portion of amino acids 320-370 of human Beta-arrestin 1 was used as the immunogen. |
Application Notes
The amino acid sequence used as immunogen is 100% homologous in human, mouse, rat, cow and zebrafish, and 70% homologous in chicken and frog. |
Genebank Info (Protein)
P49407 |
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Western blot analysis of Beta-arrestin 1 in human testis lysate in the 1) absence and 2) presence of immunizing peptide using IMG-6428A at 2.0 ug/ml. IMGENEX’s goat anti-rabbit Ig HRP secondary antibody (IMGENEX, 20301) and PicoTect ECL substrate solution (IMGENEX, 10087K) were used for this test. |
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Reference
1. Lohse, MJ. et al. Science. 248:1547-1550 (1990).
2. Buchanan, FG. et al. Proc. Nat. Acad. Sci. 103:1492-1497 (2006).
3. Kovacs, JJ. et al. Science. 320:1777-1781 (2008).
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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