|
|
|
| |
Polyclonal Antibody to CBX5 |
|
|
| |
|
|
| |
|
| Catalog No: |
IMG-6453A |
| Contents: |
100 ug in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic. |
| Isotype: |
Rabbit IgG |
| Purification: |
Protein A Chromatography |
| Species: |
Dog, Human, New World monkey, Mouse |
| Predicted React: |
Rat |
| Host: |
Rabbit |
| Application: |
Western blot analysis: 3-5 ug/ml |
| Storage: |
Store at 4°C, stable for 6 months. For long-term storage, aliquot and store at -20°C. |
Recommended
Positive Control: |
HCT-116 (human) and NIH 3T3 (mouse) |
|
 |
|
|
|
|
|
|
Background
CBX5, also known as Chromobox protein homolog 5, is a regulatory protein belonging to the Polycomb group proteins family with two chromo domains. CBX5 associates with chromosomes during mitosis, specifically with chromatin during metaphase and anaphase. A component of the centromeric and pericentromeric heterochromatin, CBX5 may interact with lamin B receptor (LBR) to contribute to the association of the heterochromatin with the inner nuclear membrane. CBX5 recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. This methyl-lysine binding protein localized at heterochromatin sites is also known to mediate gene silencing and is involved in the formation of functional kinetochore through interaction with MIS12 complex proteins. |
Antigen
A portion of amino acids 50-100 of human CBX5 was used as the immunogen. |
Application Notes
The amino acid sequence used as immunogen is 100% homologous in human (isoform CRA_a), monkey and dog and 93% homologous in mouse and rat. |
Genebank Info (Protein)
P45973 |
| |
|
Western blot analysis of CBX5 in HCT-116 cell lysate in the 1) absence and 2) presence of immunizing peptide and 3) NIH 3T3 cell lysate using IMG-6453A at 3 ug/ml. IMGENEX’s goat anti-rabbit Ig HRP secondary antibody (IMGENEX, 20301) and PicoTect ECL substrate solution (IMGENEX, 10087K) were used for this test. |
|
|
|
|
|
| - |
 |
|
Reference
1. Bannister, AJ. et al. Nature. 410:120-124 (2001).
2. Hall, IM. et al. Science. 297:2232-2237 (2002).
3. Smallwood, A. et al. Genes Dev. 21:1169-1178 (2007).
|
|
|
|
Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
|
|
|
|
|
