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Product : Antibody >> New Antibodies , Stem Cell Markers >> CBX5
      
 

Polyclonal Antibody to CBX5

 
Cat. No.DescriptionFormatUnitPrice(USD)QuantityBulk Quotation
IMG-6453A
CBX5Purified
0.1 mg
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Catalog No: IMG-6453A
Imgenex is now a part of Novus Biologicals! All orders, billing and shipments will be received from Novus. Please contact Novus directly for any questions – novus@novusbio.com. To insure proper processing of you order, please insure that your purchase orders show Novus Biologicals as the vendor.
Contents: 100 ug in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Isotype: Rabbit IgG
Purification: Protein A Chromatography
Species: Dog, Human, New World monkey, Mouse
Predicted React: Rat
Host: Rabbit
Application: Western blot analysis: 3-5 ug/ml
Storage: Store at 4°C, stable for 6 months. For long-term storage, aliquot and store at -20°C.
Recommended
Positive Control:
HCT-116 (human) and NIH 3T3 (mouse)
 

Background
CBX5, also known as Chromobox protein homolog 5, is a regulatory protein belonging to the Polycomb group proteins family with two chromo domains. CBX5 associates with chromosomes during mitosis, specifically with chromatin during metaphase and anaphase. A component of the centromeric and pericentromeric heterochromatin, CBX5 may interact with lamin B receptor (LBR) to contribute to the association of the heterochromatin with the inner nuclear membrane. CBX5 recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. This methyl-lysine binding protein localized at heterochromatin sites is also known to mediate gene silencing and is involved in the formation of functional kinetochore through interaction with MIS12 complex proteins.

Antigen
A portion of amino acids 50-100 of human CBX5 was used as the immunogen.

Application Notes
The amino acid sequence used as immunogen is 100% homologous in human (isoform CRA_a), monkey and dog and 93% homologous in mouse and rat.

Genebank Info (Protein): P45973
Gene ID: 23468
SwissProt: P45973
 
CBX5 Antibody

Western blot analysis of CBX5 in HCT-116 cell lysate in the 1) absence and 2) presence of immunizing peptide and 3) NIH 3T3 cell lysate using IMG-6453A at 3 ug/ml.  IMGENEX’s goat anti-rabbit Ig HRP secondary antibody (IMGENEX, 20301) and PicoTect ECL substrate solution (IMGENEX, 10087K) were used for this test.

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Reference
1. Bannister, AJ. et al. Nature. 410:120-124 (2001).
2. Hall, IM. et al. Science. 297:2232-2237 (2002).
3. Smallwood, A. et al. Genes Dev. 21:1169-1178 (2007).


Research purposes only. Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.


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