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Polyclonal Antibody to TSC22 |
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| Catalog No: |
IMG-6454A |
| Contents: |
100 ug in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic. |
| Isotype: |
Rabbit IgG |
| Purification: |
Protein A Chromatography |
| Species: |
Dog, Human, Mouse, Rat |
| Predicted React: |
Chicken, Opossum, Pufferfish, Zebrafish |
| Host: |
Rabbit |
| Application: |
Western blot analysis: 3-5 ug/ml |
| Storage: |
Store at 4°C, stable for 6 months. For long-term storage, aliquot and store at -20°C. |
Recommended
Positive Control: |
Jurkat (human) and RAW (mouse) |
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Background
TSC22 domain family protein, or TSC22, is a transcription factor that belongs to the large family of early response genes. This transcriptional repressor is known to act on the C-type natriuretic peptide (CNP) promoter. TSC22 belongs to the TSC-22/Dip/Bun family and is an intracellular protein that may be found in the cytoplasm or nucleus. This transcription factor is known to regulate cell growth, differentiation and cell death and is involved in modulating the transcriptional activity of Smad3 and Smad4. This protein is ubiquitously expressed in most tissues and widely in both fetal and adult tissues. It is generally expressed in aortic endothelial cells, and induced by cytokines, including TGFB. These proteins may be possible therapeutic targets of leukemia and prostate cancer. |
Antigen
A portion of amino acids 100-150 of human TSC22 was used as the immunogen. |
Application Notes
The amino acid sequence used as immunogen is 100% homologous in human, mouse, rat, and dog, 94% homologous in chicken, opossum and pufferfish and 83% homologous in zebrafish. |
Genebank Info (Protein)
NP_904358 |
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Western blot analysis of TSC22 in Jurkat cell lysate in the 1) absence and 2) presence of immunizing peptide and 3) RAW cell lysate using IMG-6454A at 3 ug/ml. IMGENEX’s goat anti-rabbit Ig HRP secondary antibody (IMGENEX, 20301) and PicoTect ECL substrate solution (IMGENEX, 10087K) were used for this test. |
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Reference
1. Jay, P. et al. Biochem. Biophys. Res. Commun. 222:821-826 (1996).
2. Kester, HA. et al. J. Biol. Chem. 274:27439-27447 (1999).
3. Ohta, S. et al. Europ. J. Biochem. 242:460-466 (1996).
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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