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Product : Antibody >> Antigen Affinity Purified , Chromatin , DNA Methylation & Rep.. , New Antibodies >> Dnmt3a
      
 

Peptide-affinity Purified Polyclonal Antibody to Dnmt3a

 
Cat. No.DescriptionFormatUnitPrice(USD)QuantityBulk Quotation
IMG-6465A
Dnmt3aPurified, Peptide Affinity
0.1 mg
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Catalog No: IMG-6465A
Imgenex is now a part of Novus Biologicals! All orders, billing and shipments will be received from Novus. Please contact Novus directly for any questions – novus@novusbio.com. To insure proper processing of you order, please insure that your purchase orders show Novus Biologicals as the vendor.
Contents: 100 ug in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Isotype: Rabbit Ig
Purification: Antigen Affinity Chromatography
Species: Chimpanzee, Human, Rhesus Monkey
Predicted React: Cat, Cow, Horse, Mouse, Rat
Host: Rabbit
Application: Western blot analysis: 1-3 ug/ml
Storage: Store at 4°C, stable for 6 months. For long-term storage, aliquot and store at -20°C.
Recommended
Positive Control:
Dnmt3a transfected cell lysate (IMGENEX, 40212)
 

Background
Dnmt3a, also known as DNA (cytosine-5)-methyltransferase 3A, is a nuclear protein belonging to the C5-methyltransferase family with an ADD-type zinc finger and a PWWP domain. This nuclear protein localizes to euchromatin and is essential for genome wide de novo methylation and is also essential for development. DNA methylation is coordinated with methylation of histones. Dnmt3a associates with HDAC1 through its ADD-type zinc-finger. It binds to the ZNF238 transcriptional repressor and is also known to interact with other DNA methyltransferases like Dnmt1 and Dnmt3b.

Antigen
A portion of amino acids 10-50 of human Dnmt3a was used as the immunogen.

Application Notes
This antibody will not cross react with Dnmt3b.

The amino acid sequence used as immunogen is 100% homologous in human (isoforms CRA_a, CRA_b and CRA_c), chimpanzee and rhesus monkey, 85% homologous in horse and cat, 76% homologous in cow and 71% homologous in mouse and rat.

Genebank Info (Protein): NP_072046.2
Gene ID: 1788
SwissProt: Q9Y6K1
 
Dnmt3a Antibody

Western blot analysis of Dnmt3a in Dnmt3a transfected 293 cell lysate (IMGENEX, 40212) using IMG-6465A at 1 ug/ml. IMGENEX’s goat anti-rabbit Ig HRP secondary antibody (IMGENEX, 20301) and PicoTect ECL substrate solution (IMGENEX, 10087K) were used for this test.

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Reference
1. Chen, T. et al. J. Biol. Chem. 277:38746-38754 (2002).
2. Schlesinger, Y. et al. Nat. Genet. 39:232-236 (2007).
3. Li H. et al. J. Biol. Chem. 281:19489-19500 (2006).


Research purposes only. Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.


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