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Peptide-affinity Purified Polyclonal Antibody to DDX4 |
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| Catalog No: |
IMG-6480A |
| Contents: |
100 ug in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic. |
| Isotype: |
Rabbit Ig |
| Purification: |
Antigen Affinity Chromatography |
| Species: |
Chimpanzee, Dog, Horse, Human, New World monkey, Mouse |
| Predicted React: |
Opossum, Rat |
| Host: |
Rabbit |
| Application: |
IHC (paraffin): 5 ug/ml |
| Storage: |
Store at 4°C, stable for 6 months. For long-term storage, aliquot and store at -20°C. |
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Background
DDX4 belongs to the DEAD box helicase family or DDX4/VASA subfamily. DEAD box proteins are characterized by the conserved Asp-Glu-Ala-Asp (DEAD) motif and are putative RNA helicases. This protein has a glycine-rich N terminus with multiple repeats of an RGG motif, which is believed to function in RNA binding. They have a role in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. The members of this family are involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a homolog of VASA proteins in Drosophila. It is specifically expressed in the germ cell lineage in both sexes and functions in germ cell development. It is required during gametogenesis in the adult and in the specification of the germ cell lineage during embryogenesis. This is conserved in invertebrate and vertebrate species. |
Antigen
A portion of amino acids 700-724 of DDX4 was used as the immunogen. |
Application Notes
The amino acid sequence used as immunogen is 100% homologous in human, mouse, chimpanzee, horse, dog and monkey and 97% homologous in rat and opossum. |
Genebank Info (Protein)
AF262962 |
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Immunohistochemical analysis of DDX4 in formalin-fixed, paraffin-embedded mouse testis tissue using an isotype control (top left) and IMG-6480A (bottom left, right) at 5 ug/ml. |
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Reference
1. Abe, K. et al. Mammalian Genome. 8:622-623 (1997).
2. Castrillion, D. et al. Proc. Nat. Acad. Sci. 97:9585-9590 (2000).
3. Chu, D. et al. Nature. 443:101-105 (2006).
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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