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Product : Antibody >> Antigen Affinity Purified , New Antibodies , Stem Cell Markers >> DDX4
      
 

Peptide-affinity Purified Polyclonal Antibody to DDX4

 
Cat. No.DescriptionFormatUnitPrice(USD)QuantityBulk Quotation
IMG-6480A
DDX4Purified, Peptide Affinity
0.1 mg
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Catalog No: IMG-6480A
Imgenex is now a part of Novus Biologicals! All orders, billing and shipments will be received from Novus. Please contact Novus directly for any questions – novus@novusbio.com. To insure proper processing of you order, please insure that your purchase orders show Novus Biologicals as the vendor.
Contents: 100 ug in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Isotype: Rabbit Ig
Purification: Antigen Affinity Chromatography
Species: Chimpanzee, Dog, Horse, Human, New World monkey, Mouse
Predicted React: Opossum, Rat
Host: Rabbit
Application: IHC (paraffin): 5 ug/ml
Storage: Store at 4°C, stable for 6 months. For long-term storage, aliquot and store at -20°C.
 

Background

DDX4 belongs to the DEAD box helicase family or DDX4/VASA subfamily. DEAD box proteins are characterized by the conserved Asp-Glu-Ala-Asp (DEAD) motif and are putative RNA helicases. This protein has a glycine-rich N terminus with multiple repeats of an RGG motif, which is believed to function in RNA binding. They have a role in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. The members of this family are involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a homolog of VASA proteins in Drosophila. It is specifically expressed in the germ cell lineage in both sexes and functions in germ cell development. It is required during gametogenesis in the adult and in the specification of the germ cell lineage during embryogenesis. This is conserved in invertebrate and vertebrate species.

Antigen
A portion of amino acids 700-724 of DDX4 was used as the immunogen.

Application Notes
The amino acid sequence used as immunogen is 100% homologous in human, mouse, chimpanzee, horse, dog and monkey and 97% homologous in rat and opossum.

Genebank Info (Protein): AF262962
 
DDX4

Immunohistochemical analysis of DDX4 in formalin-fixed, paraffin-embedded mouse testis tissue using an isotype control (top left) and IMG-6480A (bottom left, right) at 5 ug/ml.

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Reference
1. Abe, K. et al. Mammalian Genome. 8:622-623 (1997).
2. Castrillion, D. et al. Proc. Nat. Acad. Sci. 97:9585-9590 (2000).
3. Chu, D. et al. Nature. 443:101-105 (2006).


Research purposes only. Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.


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