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Product : Antibody >> Development & Differe.. , IHCProTM , Immunohistochemistry , Stem Cell Markers >> CDX2 / CDX3
      
 

Peptide-affinity Purified Polyclonal Antibody to CDX2 /CDX3

 
Cat. No.DescriptionFormatUnitPrice(USD)QuantityBulk Quotation
IMG-6520A
CDX2 / CDX3Purified, Peptide Affinity
0.1 mg
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Catalog No: IMG-6520A
Imgenex is now a part of Novus Biologicals! All orders, billing and shipments will be received from Novus. Please contact Novus directly for any questions – novus@novusbio.com. To insure proper processing of you order, please insure that your purchase orders show Novus Biologicals as the vendor.
Contents: 100 ug of antibody in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Isotype: Rabbit Ig
Purification: Peptide affinity chromatography was used to purify the CDX2 antibody
Species: Cow, Horse, Human, Mouse, Opossum, Rat, Rhesus Monkey
Predicted React: Chicken
Host: Rabbit
Application: IHC (p): 10 ug/ml
Western blot analysis: 1-3 ug/ml
Storage: Store CDX2 antibody at 4°C, stable for 6 months. For long-term storage, aliquot antibody and store at -20°C.
 

Background
CDX2 is a homeobox protein that binds to the A/T rich sequence in the insulin promoter to stimulate transcription. It is a caudal type homeobox gene that encodes an intestine-specific transcription factor that is expressed early in intestinal development. The CDX2 protein is expressed in primary and metastatic colorectal carcinomas and has also been demonstrated in the intestinal metaplasia of the stomach and intestinal-type gastric cancer. It also plays an important role in maintenance of epithelial lining of the intestines.

Antigen
A portion of amino acids 1-60 of human CDX2 /CDX3 was used as the immunogen for the antibody.

Application Notes
The amino acid sequence used as immunogen for the CDX2 /CDX3 antibody is 100% homologous in human, horse, rhesus monkey, opossum, mouse, cattle and rat, 78% homologous in bowfin, and 75% in chicken.

Genebank Info (Protein): NP_001256.2
Gene ID: 1045
SwissProt: Q99626
 
CDX2 CDX3 Antibody WB

Western blot analysis of HCT-116 lysate in the 1) absence and 2) presence of immunizing peptide using CDX2 / CDX3 antibody at 2 ug/ml. IMGENEX’s goat anti-rabbit Ig HRP secondary antibody (IMGENEX, 20301) and PicoTect ECL substrate solution (IMGENEX, 10087K) were used for this test.

CDX2 CDX3 Antibody IHC

Formalin-fixed, paraffin-embedded human spleen probed with CDX2 / CDX3 antibody at 5 ug/ml.

Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.

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Reference
1. Alkhoury, S. et al. Am. J. Physiol. Gastrointest. Liver Physiol. 289:G285-G290 (2005).
2. Chase, A. et al. Blood 93:1025-1031 (1999).
3. Kazumori, H. et al. Gut. 58:620-628 (2009).


Research purposes only. Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.


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