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Peptide-affinity Purified Polyclonal Antibody to FLII (FLIIH) |
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| Catalog No: |
IMG-6527A |
| Contents: |
100 ug antibody in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic. |
| Isotype: |
Rabbit Ig |
| Purification: |
Peptide affinity chromatography |
| Species: |
Chimpanzee, Horse, Human, Mouse, Rat, Rhesus Monkey |
| Predicted React: |
Dog, Cow, Chicken |
| Host: |
Rabbit |
| Application: |
IHC (paraffin): 5 ug/ml |
| Storage: |
Store FLII antibody at 4°C, stable for 6 months. For long-term storage, aliquot and store antibody at -20°C. |
Recommended
Positive Control: |
Brain |
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Background
FLII (Flightless-1 homolog, FLIIH) is a 1269 amino acid containing protein with a gelsolin-like actin binding domain and an N-terminal leucine rich protein-protein interaction domain acts as a coactivator in transcriptional activation by hormone-activated nuclear receptors (NR) and in conjunction with NCOA2 and CARM1. FLII is involved in estrogen hormone signaling and early embryonic development. The protein also plays a role in regulation of cytoskeletal rearrangements involved in cytokinesis and cell migration. Deletion of the FLII gene may be a cause of Smith-Magenis syndrome (SMS). |
Antigen
A portion of amino acids 90-140 of human FLIIH was used as the immunogen for this antibody. |
Application Notes
The amino acid sequence used as immunogen is 100% homologous in human, rhesus monkey, horse, mouse, rat and chimpanzee, 94% homologous in cattle and dog, and 89% homologous in chicken. |
| Genebank Info (Protein): NP_002009.1 |
| Gene ID: 2314 |
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Immunohistochemical analysis of FLII using FLII polyclonal antibody. Formalin-fixed, paraffin-embedded human brain tissue probed with an isotype control antibody (left) and FLII antibody (right) at 5 ug/ml. |
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Reference
1. Sugiyama, S. et al. Dev. Growth Differ. 51:369-377 (2009).
2. Xu, J. et al. J. Biol. Chem. 284:14377-14385 (2009).
3. Ramakrishnan, R. et al. Oncogene 23:7087-7094 (2004).
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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