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Polyclonal Antibody to 14-3-3 zeta |
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| Catalog No: |
IMG-6530A |
| Contents: |
100 ug in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic. |
| Isotype: |
Rabbit IgG |
| Purification: |
Protein A Chromatography |
| Species: |
Chimpanzee, Cow, Dog, Human, Mouse, Opossum, Pig |
| Predicted React: |
Frog |
| Host: |
Rabbit |
| Application: |
IHC (paraffin): 5 ug/ml
Western blot analysis: 3-5 ug/ml |
| Storage: |
Store at 4°C, stable for 6 months. For long-term storage, aliquot and store at -20°C. |
Recommended
Positive Control: |
Brain |
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Background
14-3-3 zeta is a 245 amino acid containing protein belonging to the 14-3-3 family mediating signal transduction by binding to phosphoserine containing proteins. It is a highly conserved protein in both plants and mammals. The encoded protein interacts with IRS1 implicating a plausible role in regulating insulin sensitivity. There are several transcript variants, with seven known mammalian isoforms: alpha, beta, gamma, delta, epsilon, zeta and eta. 14-3-3 proteins function in broad regulation of proteins by cytoplasmic sequestration, occupation of interaction domains and import/export sequences, prevention of degradation, and activation/repression of enzyme activity. The protein is highly abundant in brain. |
Antigen
A portion of amino acids 50-100 of human 14-3-3 zeta was used as the immunogen. |
Application Notes
The amino acid sequence used as immunogen is 100% homologous in human, cattle, rat, mouse, chimpanzee, rhesus monkey, opossum, pig, chicken and dog, and 94% homologous in goldfish. |
Genebank Info (Protein)
NP_001129174.1 |
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Western blot analysis of 14-3-3 zeta in human brain lysate in the 1) absence, 2) presence of immunizing peptide, 3) mouse brain lysate and 4) rat brain lysate using IMG-6530A at 4 ug/ml. IMGENEX’s goat anti-rabbit Ig HRP secondary antibody (IMGENEX, 20301) and PicoTect ECL substrate solution (IMGENEX, 10087K) were used for this test. |
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Immunohistochemical analysis of 14-3-3 zeta in formalin-fixed, paraffin-embedded human brain tissue using an isotype control (top) and IMG-6530A (bottom) at 5 ug/ml. |
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Reference
1. Murthi, P. et al. Placenta 29:798-801 (2008).
2. Frasor, J. et al. Cancer Res. 66:7334-7340 (2006).
3. Heidenblad, M. et al. BMC Med. Genomics 31:3 (2008).
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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