Monoclonal Antibody to SARS-Nucleocapsid protein

Background
It has recently been shown that SARS is caused by a human coronavirus. Human coronaviruses are the major cause of upper respiratory tract illness in humans, such as the common cold. Coronaviruses are positive-stranded RNA viruses, featuring the largest viral RNA genomes known to date (27-31 kb). The first step in coronavirus infection is binding of the viral spike protein, a 139-kDa protein, to certain receptors on host cells. The spike protein is the main surface antigen of the coronavirus. The most prominent protein in the culture supernatants infected with SARS virus is a 46 kDa nucleocapsid protein. This suggests that the nucleocapsid protein is a major immunogen that may be useful for early diagnostics.

Antigen
The antibody was developed by immunizing mice with full-length recombinant SARS nucleocapsid protein (Genbank accession no. NP_828858). The specific epitope has not been determined, however it is known to be different than IMG-654.

Application Notes
A 46 kDa band should be observed.

Genebank Info (Protein)
NP_006504

Reference
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4. Krokhin O, Li Y, Andonov A, Feldmann H, Flick R, Jones S, Stroeher U. Mol Cell Proteomics. May;2(5):346-356 (2003).