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Product : Antibody >> IHCProTM , Immunohistochemistry , Stem Cell Markers >> DPPA4
      
 

Polyclonal Antibody to DPPA4

 
Cat. No.DescriptionFormatUnitPrice(USD)QuantityBulk Quotation
IMG-6571A
DPPA4Purified
0.1 mg
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Catalog No: IMG-6571A
Imgenex is now a part of Novus Biologicals! All orders, billing and shipments will be received from Novus. Please contact Novus directly for any questions – novus@novusbio.com. To insure proper processing of you order, please insure that your purchase orders show Novus Biologicals as the vendor.
Contents: 100 ug in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Isotype: Rabbit IgG
Purification: Protein A chromatography was used to purify the DPPA4 antibody.
Species: Chimpanzee, Human, Mouse
Predicted React: New World monkey
Host: Rabbit
Application: IHC (paraffin): 5-10 ug/ml
Western blot analysis: 2-4 ug/ml
Storage: Store DPP4A antibody at 4°C, stable for 6 months. For long-term storage, aliquot antibody and store at -20°C.
 

Background
Developmental pluripotency associated 4 (DPPA4) protein is abundantly found in embryonic stem cells and in early embryos. DPPA4 protein is localized in the embryonic stem cell nucleus associated with transcriptionally active chromatin. The Dppa4 gene overexpression does not support embryonic stem cell self-renewal but partially inhibits embryonic stem cell differentiation.  Suppression of DPPA4 expression by short hairpin RNA induced embryonic stem cell into a primitive ectoderm lineage.

Antigen
A portion of amino acid 1 to 50 of human DPPA4 was used as immunogen for the antibody.

Application Notes
The amino acid sequence used as immunogen for the DPPA4 antibody is 100% homologous in human, chimpanzee and 72% homologous in monkey.

Genebank Info (Protein): Q7L190
Gene ID: 55211
SwissProt: Q7L190
 
DPPA4 Antibody WB

Western blot analysis human testis lysate using 1) pre-bleed and 2) DPPA4 antibody at 2 ug/ml. IMGENEX’s goat anti-rabbit Ig HRP secondary antibody (IMGENEX, 20301) and PicoTect ECL substrate solution (IMGENEX, 10087K) were used for this test.

DPPA4 Antibody IHC

Formalin-fixed, paraffin-embedded human placenta stained with DPPA4 antibody at 10 ug/ml.

Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.

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Reference
1. Masaki, H. et al. The Journal of Biol. Chem. 282:33034-33042 (2007).
2. Maldonado-Saldivia, J. et al. Stem Cells. 25:19-28 (2007).
3. Bortvin, A. et al. Development. 130:1673-1680 (2003).


Research purposes only. Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.


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