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Monoclonal Antibody to IL-17A (Clone 4H1524) Alexa Fluor® 488 Conjugate |
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| Catalog No: |
IMG-6580AF488 |
| Contents: |
IL-17A antibody in PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic. |
| Isotype: |
Mouse IgG2b, Kappa |
| Clone: |
4H1524 |
| Purification: |
Protein G Chromatography |
| Species: |
Human |
| Host: |
Mouse |
| Application: |
Flow (Intracellular): 10 ul/10^6 cells (0.25 ug) |
| Storage: |
Store at 4°C, stable for 6 months. DO NOT FREEZE; MATERIAL IS LIGHT-SENSITIVE. |
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Background
Interleukin 17 (IL-17) is a pro-inflammatory cytokine produced by a subset of T helper cells that develops distinct from the Th1- and Th2- cell differentiation pathways. IL-17, also known as CTLA-8, stimulates induction of other pro-inflammatory cytokines TNF alpha, IL-1 beta, IL-6, and IL-8, and reports strongly suggest the involvement of IL-17 in several chronic inflammatory diseases such as rheumatoid arthritis, psoriasis and multiple sclerosis. TGF-ß (differentiation) and IL-23 (expansion) are required for induction and maintenance of Th17 (IL-17 producing) cells, which in turn induce the other pro-inflammatory cytokines. IL-17 (~32kDa) protein is produced and exists as a homo-dimer, has homology to a herpes virus early protein, is one of the six members (IL-17A-F) of this cytokine family, and is well characterzed and highly expressed by activated effector memory T cells. |
Antigen
IMGENEX's full length recombinant human IL-17A protein (IMR-295-0005) was used as the immunogen. |
Genebank Info (Protein)
AAH66253 |
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Intracellular flow cytometric analysis of IL-17A in human PBMCs (lymphocytes) stimulated with PMA (5 ng/ml), Ionomycin (1 mM), and Brefeldin A (1X) for 5 hrs, using CD4+ cells and analyzed with IMG-6580AF488 (red) at 10 ul/10^6 cells (green represents isotype control). IMGENEX's intracellular flow kit (10083K) was used for this test. |
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Reference
1. Chen Z and O’Shea JJ 2008 Immunol Res 41: 87-102
2. Mangan PR et al 2006 Nature 441: 231-234
3. Veldhoen M et al 2006 Immunity 24:179-189
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for six months from the date of purchase.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc. IMGENEX sells Alexa Fluor® dye conjugated antibodies under license from Molecular Probes, Inc. for research use only.
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