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Monoclonal Antibody to IL-17A (Clone 4H1524) Alexa Fluor® 488 Conjugate

 
Cat. No.DescriptionFormatUnitPrice(USD)QuantityBulk Quotation
IMG-6580AF488-25
IMG-6580AF488-100
IL-17AAlexa Fluor® 488
25 Tests (250 ul)
100 Tests (1 ml)
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Catalog No: IMG-6580AF488
Imgenex is now a part of Novus Biologicals! All orders, billing and shipments will be received from Novus. Please contact Novus directly for any questions – novus@novusbio.com. To insure proper processing of you order, please insure that your purchase orders show Novus Biologicals as the vendor.
Contents: IL-17A antibody in PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Isotype: Mouse IgG2b, Kappa
Clone: 4H1524
Purification: Protein G Chromatography
Species: Human
Host: Mouse
Application: Flow (Intracellular): 10 ul/10^6 cells (0.25 ug)
Storage: Store at 4°C, stable for 6 months. DO NOT FREEZE; MATERIAL IS LIGHT-SENSITIVE.
 

Background
Interleukin 17 (IL-17) is a pro-inflammatory cytokine produced by a subset of T helper cells that develops distinct from the Th1- and Th2- cell differentiation pathways. IL-17, also known as CTLA-8, stimulates induction of other pro-inflammatory cytokines TNF alpha, IL-1 beta, IL-6, and IL-8, and reports strongly suggest the involvement of IL-17 in several chronic inflammatory diseases such as rheumatoid arthritis, psoriasis and multiple sclerosis. TGF-ß (differentiation) and IL-23 (expansion) are required for induction and maintenance of Th17 (IL-17 producing) cells, which in turn induce the other pro-inflammatory cytokines. IL-17 (~32kDa) protein is produced and exists as a homo-dimer, has homology to a herpes virus early protein, is one of the six members (IL-17A-F) of this cytokine family, and is well characterzed and highly expressed by activated effector memory T cells.

Antigen
IMGENEX's full length recombinant human IL-17A protein (IMR-295-0005) was used as the immunogen.

Genebank Info (Protein): NP_002181
Gene ID: 3605
SwissProt: Q16552
 
IL-17A Antibody IMG-6580AF488-25

Intracellular flow cytometric analysis of IL-17A in human PBMCs (lymphocytes) stimulated with PMA (5 ng/ml), Ionomycin (1 mM), and Brefeldin A (1X) for 5 hrs, using CD4+ cells and analyzed with IMG-6580AF488 (red) at 10 ul/10^6 cells (green represents isotype control). IMGENEX's intracellular flow kit (10083K) was used for this test.

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Reference
1. Chen Z and O’Shea JJ 2008 Immunol Res 41: 87-102
2. Mangan PR et al 2006 Nature 441: 231-234
3. Veldhoen M et al 2006 Immunity 24:179-189


Research purposes only. Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for six months from the date of purchase.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc. IMGENEX sells Alexa Fluor® dye conjugated antibodies under license from Molecular Probes, Inc. for research use only.

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