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Monoclonal Antibody to MyD88 (clone 4D6) Alexa Fluor® 488 Conjugate |
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| Catalog No: |
IMG-6625AF488 |
| Contents: |
25 tests in 250 µl PBS containing 0.05% BSA and 0.05% sodium azide. Myd88 monoclonal antibody contains sodium azide which is highly toxic. |
| Isotype: |
Mouse IgG1, Kappa |
| Clone: |
4D6 |
| Purification: |
Protein G Chromatography |
| Species: |
Human |
| Predicted React: |
Mouse, Rat |
| Host: |
Mouse |
| Application: |
Flow (Intracellular): 10 ul/test/10^6 cells (0.5 ug) |
| Storage: |
Store at 4°C, stable for 6 months. DO NOT FREEZE; MATERIAL IS LIGHT-SENSITIVE. |
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Background
MyD88, a protein involved in interleukin-1 (IL-1) mediated signalling was originally isolated as myeloid differentiation primary response gene (3,4). MyD88 possesses a N-terminal death domain similar to cytoplasmic segments of TNF receptor 1, Fas, and C-terminal region related to IL-1 and Toll receptors. Overexpression of MyD88 induces activation the c-Jun N-terminal kinase and NF-kB through its death domain (1,2). The C-terminus of MyD88 interacts with the IL-1 receptor and blocks NF-kB activation induced by IL-1, but not by NF-kB. |
Antigen
A portion of amino acids 50-100 of human MyD88 was used as the immunogen. |
Application Notes
The amino acid sequence used as immunogen is 100% homologous in human (isoform 1: 317 amino acids, isoform 3: 264 amino acids, isoform 4: 204 amino acids; isoform 5: 159 amino acids, CRA_a: 304 amino acids, CRA_b: 309 amino acids), gorilla, chimp, rhesus monkey and orangutan, 82% homologous in horse, 73% homologous in dog, 68% homologous in cat and mouse (isoform CRA_a: 314 amino acids, CRA_b: 177 aa), and 63% homologous in rat. |
Genebank Info (Protein)
NP_002459 |
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Intracellular flow cytometric analysis of MyD88 antibody in Jurkat cells using 10 ul (0.5 ug) of Myd88 antibody. Green represents isotype control (20108 ); red represents anti-MyD88 antibody. An intracellular flow kit (10083K) was used for this test. |
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Reference
1. Bonnert TP, Garka KE, Parnet P, Sonoda G, Testa JR, and Sims JE. The cloning and characterization of human MyD88: a member of an IL-1 receptor related family. FEBS Lett 402: 81-84 (1997).
2. Hardiman G., Rock FL, Balsubramanian S, Kastelein RA, and Bazan, JF. Molecular characterization and molecular analysis of human MyD88. Oncogene 13 (11): 2467-2475 (1996).
3. Burns K, Martino F, Esslinger C, Pahl H, Schneider P, Bodmer JL, Di Marco F, French I, and Tschopp J. MyD88, an adapter protein involved in interleukin-1 signaling. J Biol Chem 273 (20): 12203-12209 (1998).
4. Wesche H, Henzel WJ, Shillinglaw W, Li S, and Can, Z. MyD88: an adapter recruits IRAK to the IL-1 receptor complex. Immunity 7 (6): 837-847 (1997).
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for six months from the date of purchase.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc. IMGENEX sells Alexa Fluor® dye conjugated antibodies under license from Molecular Probes, Inc. for research use only.
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