Monoclonal Antibody to TLR5 (Toll-like receptor 5)

Background
The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules and could constitute an important and unrecognized component of innate immunity in humans. The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition. TLRs characterized so far activate the MyD88/interleukin-1 receptor-associated kinase (IRAK) signaling pathway. Thirteen homologs of TLRs (TLR1-13) have been described. Toll-like receptor 5 (TLR5) expression is upregulated following exposure to bacteria or to the TLR5 agonist, flagellin. Gram-negative bacteria, stimulate monocyte/macrophage cells in a TLR5-specific, CD14-independent manner. The TLR5 receptor thus appears to be the principal means by which the innate immune system recognizes flagellated bacterial pathogens. 

Antigen
This antibody was developed against KLH-conjugated synthetic peptide corresponding to a portion of human TLR5 between amino acids 700-800. The antibody also reacts with mouse TLR5.

Application Notes
This antibody recognizes an intracellular epitope in the cytoplasmic domain of TLR5.

View all TLR5 antibodies

Genebank Info (Protein)
NP_003259

Reference
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4. Zhenyu Cai, Amir Sanchez, Zhongcheng Shi, Tingting Zhang, Mingyao Liu, Dekai Zhang. Activation of Toll-Like Receptor 5 on Breast Cancer Cells by Flagellin Suppresses Cell Proliferation and Tumor Growth. Cancer Res. 2011 April 1; 71(7): 2466–2475.
5. Cesar F. Ortega-Cava, Shunji Ishihara, Mohammad A. K. Rumi, M. M. Aziz, Hideaki Kazumori, Takafumi Yuki, Yoshiyuki Mishima, Ichiro Moriyama, Chikara Kadota, Naoki Oshima, Yuji Amano, Yasunori Kadowaki, Norihisa Ishimura, Yoshikazu Kinoshita. Epithelial Toll-Like Receptor 5 Is Constitutively Localized in the Mouse Cecum and Exhibits Distinctive Down-Regulation during Experimental Colitis. Clin Vaccine Immunol. 2006 January; 13(1): 132–138.
6. Vincent Feuillet, Samir Medjane, Isabelle Mondor, Olivier Demaria, Philippe P. Pagni, Jorge E. Galán, Richard A. Flavell, Lena Alexopoulou. Involvement of Toll-like receptor 5 in the recognition of flagellated bacteria. Proc Natl Acad Sci U S A. 2006 August 15; 103(33): 12487–12492.