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Polyclonal Antibody to HMGB1 |
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| Catalog No: |
IMG-6666A |
| Contents: |
100 ug antibody in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic. |
| Isotype: |
Rabbit IgG |
| Purification: |
Protein A Chromatography |
| Species: |
Chicken, Human, Mouse, New World monkey, Rat |
| Host: |
Rabbit |
| Application: |
Western blot analysis: 5-10 ug/ml |
| Storage: |
Store antibody at 4°C, stable for 6 months. For long-term storage, aliquot and store at -20°C. |
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Background
HMGB1 (high mobility group protein B1) belongs to the HMGB family and contains two HMG box DNA-binding domains. It is a highly conserved, ubiquitous protein present in the nuclei and cytoplasm of nearly all cell types, and is a necessary and sufficient mediator of inflammation during sterile and infection-associated responses. HMGB1 also act as DNA nuclear binding protein that has recently been shown to be an early trigger of sterile inflammation in animal models of trauma-hemorrhage via the activation of the Toll-like receptor 4 (TLR4) and the receptor for the advanced glycation endproducts (RAGE). It is reported that the level of HMGB1 is elevated during sterile tissue injury, infection, lethal endotoxemia or sepsis, collagen-induced arthritis, and ischemia-reperfusion induced tissue injury. |
Antigen
A portion of amino acids 25-75 of human HMGB1 was used as the immunogen. |
Application Notes
The HMGB1 amino acid sequence used as immunogen is 100% homologous in human, mouse, rat, chicken, and monkey.
This antibody recognizes both A box domain (amino acids 9-79) and full length HMGB1 protein. Minimal staining in IHC and ELISPOT indicates that this antibody preferentially detects the reduced form of HMGB1. |
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Western blot analysis using HMGB1 antibody. Mouse NIH 3T3 cell lysate was probed with HMGB1 antibody at 5 ug/ml. IMGENEX's goat anti-rabbit Ig HRP secondary antibody (20301) and PicoTect ECL substrate solution (10087K) were used for this test. |
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Reference
1. Yang, H. et al. Biochim. Biophys. Acta. 1799:149-156 (2010).
2. Cohen, MJ. et al. Crit. Care. 13:R174 (2009).
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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