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Product : Antibody >> IHCProTM , Immunohistochemistry , Signal Transduction , Stem Cell Markers >> AGR2 (Anterior Gradient 2 Homolog)
      
 

Monoclonal Antibody to AGR2 (Anterior Gradient 2 Homolog)

 
Cat. No.DescriptionFormatUnitPrice(USD)QuantityBulk Quotation
IMG-6672A
AGR2 (Anterior Gradient 2 Homolog)Purified
0.1 mg
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Catalog No: IMG-6672A
Imgenex is now a part of Novus Biologicals! All orders, billing and shipments will be received from Novus. Please contact Novus directly for any questions – novus@novusbio.com. To insure proper processing of you order, please insure that your purchase orders show Novus Biologicals as the vendor.
Contents: 100 ug in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Isotype: Mouse IgG1, Kappa
Clone: IMG10E2    
Purification: Protein G Chromatography was used to purify the AGR2 antibody.
Species: Bovine, Dog, Horse, Human, Mouse, Orangutan, Panda, Platypus
Predicted React: Chicken
Host: Mouse
Application: IHC (paraffin): 5-10 ug/ml
Western blot analysis: 4-6 ug/ml
Storage: Store the AGR2 antibody at 4 deg C, stable for 6 months. For long-term storage, aliquot antibody and store at -20 deg C.
 

Background
hAG-2 and hAG-3 are recently discovered human homologues of the secreted Xenopus laevis proteins XAG-1/2 (AGR1 and AGR2) that are expressed in the cement gland, an ectodermal organ in the head associated with anteroposterior fate determination during early development(1). Although the roles of hAG-2 and hAG-3 in mammalian cells are unknown, both proteins share a high degree of protein sequence homology and lie adjacent to one another on chromosome 7p21.

Antigen
A portion of amino acids 50-100 of human AGR2 was used as the immunogen for the antibody.

Application Notes
The AGR2 amino acid sequence used as immunogen for the antibody is 100% homologous in human, rhesus monkey, orangutan, dog, horse, rabbit, marmoset, panda, bovine and Xenopus, 94% homologous in rat, 88% homolgous in mouse and 83% homologous in chicken.

Genebank Info (Protein): NP_006399
Gene ID: 10551
SwissProt: O95994
 
AGR2 (Anterior Gradient 2 Homolog) Antibody WB

Western blot analysis of human stomach in the A) absense and B) presence of immunizing peptide, C) mouse stomach, D) rat stomach tissue lysate, and E) HCT-116 cell lysate using AGR2 antibody at 5 ug/ml. IMGENEX’s goat anti-mouse Ig HRP secondary antibody (IMGENEX, 20101) and PicoTect ECL substrate solution (IMGENEX, 10087K) were used for this test.

AGR 2 (Anterior Gradient 2 Homolog) Antibody IMG-6672A

Formalin-fixed, paraffin-embedded human spleen (top) and breast (bottom) tissue (IMH-343) stained with AGR2 antibody at 5 ug/ml.

Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.

AGR2 (Anterior Gradient 2 Homolog) Antibody IHC

Formalin-fixed, paraffin-embedded human small intestine tissue stained with AGR2 antibody at 10 ug/ml.

Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.

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Reference
1. Fletcher GC et al.,Br J Cancer. 2003 Feb 24;88(4):579-85.
2. Petek E et al.,Cytogenet Cell Genet. 2000;89(3-4):141-2.


Research purposes only. Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.


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