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Monoclonal Antibody to AGR2 (Anterior Gradient 2 Homolog) |
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| Catalog No: |
IMG-6672A |
| Contents: |
100 ug in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic. |
| Isotype: |
Mouse IgG1, Kappa |
| Clone: |
IMG10E2 |
| Purification: |
Protein G Chromatography |
| Species: |
Bovine, Dog, Horse, Human, Mouse, Orangutan, Panda, Platypus |
| Predicted React: |
Chicken |
| Host: |
Mouse |
| Application: |
IHC (paraffin): 5 ug/ml
Western blot analysis: 4-6 ug/ml
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| Storage: |
Store at 4 deg C, stable for 6 months. For long-term storage, store at -20 deg C. |
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Background
hAG-2 and hAG-3 are recently discovered human homologues of the secreted Xenopus laevis proteins XAG-1/2 (AGR 1 and AGR 2) that are expressed in the cement gland, an ectodermal organ in the head associated with anteroposterior fate determination during early development(1). Although the roles of hAG-2 and hAG-3 in mammalian cells are unknown, both proteins share a high degree of protein sequence homology and lie adjacent to one another on chromosome 7p21. |
Antigen
A portion of amino acids 50-100 of human AGR2 was used as the immunogen. |
Application Notes
The amino acid sequence used as immunogen is 100% homologous in human, rhesus monkey, orangutan, dog, horse, rabbit, marmoset, panda, bovine and Xenopus, 94% homologous in rat, 88% homolgous in mouse and 83% homologous in chicken. |
| Genebank Info (Protein): NP_006399 |
| Gene ID: 10551 |
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Immunohistochemical analysis of AGR2 in formalin-fixed, paraffin-embedded human stomach tissue using IMG-6672A at 5 ug/ml. |
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Western blot analysis of AGR2 in A) human stomach tissue lysate in the absense of immunizing peptide, B) human stomach tissue lysate in the presence of immunizing peptide, C) mouse stomach tissue lysate, D) rat stomach tissue lysate, and E) HCT-116 cell lysate using IMG-6672A at 5 ug/ml. IMGENEX’s goat anti-mouse Ig HRP secondary antibody (IMGENEX, 20101) and PicoTect ECL substrate solution (IMGENEX, 10087K) were used for this test. |
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Reference
1. Fletcher GC et al.,Br J Cancer. 2003 Feb 24;88(4):579-85.
2. Petek E et al.,Cytogenet Cell Genet. 2000;89(3-4):141-2.
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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