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Monoclonal Antibody to Dectin-2 (Clone IMG3D1) |
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| Catalog No: |
IMG-6682A |
| Contents: |
100 ug antibody in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic. |
| Isotype: |
Mouse IgG3, Kappa |
| Clone: |
IMG3D1 |
| Purification: |
Protein G Chromatography was used to purify the antibody. |
| Species: |
Chimpanzee, Human, Mouse, Orangutan, Rat |
| Host: |
Mouse |
| Application: |
Flow (Cell Surface): 0.5 ug/5x10^5 cells
IHC (paraffin): 5 ug/ml
Western blot analysis: 2-5 ug/ml
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| Storage: |
Store antibody at 4°C, stable for 6 months. For long-term storage, aliquot and store at -20°C. |
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Background
Dectin-2 is a C-type lectin expressed by dendritic cells, macrophages and inflammatory monocytes. It is a type II membrane receptor, with a short cytoplasmic tail, and a C-terminal C-type lectin domain. Dectin-2 is a receptor with specificity for high mannose structures and mediates cytokine production from DC and other antigen presenting cells. Though, Dectin-2 has no obvious signaling motifs attached to CP tail, reports indicate that Dectin-2 employs FcRγ for internalization and to activate NF-ķB pathway for cytokine production. Though it shares only 20-25% of homology with Dectin-1, it functions similarly as pattern recognition receptor (PRR) for fungal infections, to activate both innate (Syk-CARD9 innate signaling pathway) and adaptive immune responses (by activation of DC) to fungal infections. Thus Dectin-2 is a connector molecule of both innate and adaptive immunity functioning at DC level. |
Antigen
A portion of amino acids 75-125 of human Dectin-2 was used as the immunogen for the antibody. |
Application Notes
The amino acids sequence used as immunogen is 100% homologous in human, orangutan and chimp, 83% homologous in rhesus monkey, bovine and sheep, 65% homologous in mouse and 52% homologous in rat. |
| Genebank Info (Protein): NP_001007034.1 |
| Gene ID: 93978 |
| SwissProt: Q6EIG7 |
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Western blot analysis of Dectin-2 in human heart lysate in the 1) absence and 2) presence of immunizing peptide, 3) mouse heart lysate, 4) rat heart lysate and 5) human Ramos cell lysate using Dectin-2 antibody at 2 ug/ml. IMGENEX’s goat anti-mouse Ig HRP secondary antibody (20101) and PicoTect ECL substrate solution (10087K) were used for this test. |
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Surface staining of Dectin-2 on 5x10^5 human PBMCs using 0.5 ug of Dectin-2 antibody (red) and 0.5 ug of isotype control antibody (green). IMGENEX’s cell surface flow kit (10084K) was used for this test. |
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Formalin-fixed, paraffin-embedded normal human spleen tissue stained with Dectin-2 antibody (5 ug/ml), peroxidase-conjugate and DAB chromogen. IMGENEXs human tissue TMA (IMH-343) was used for this test. |
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Reference
1. Flornes LM, Bryceson YT, Spurkland A, Lorentzen JC, Dissen E, Fossum S. Identification of lectin-like receptors expressed by antigen presenting cells and neutrophils and their mapping to a novel gene complex. Immunogenetics, 56(7):506-517 (2004).
2. Kanazawa N, Tashiro K, Inaba K, Lutz MB, Miyachi Y. Molecular cloning of human dectin-2. J Invest Dermatol. 122(6):1522-1524 (2004).
3. Willment JA, Brown GD. C-type lectin receptors in antifungal immunity. Trends Microbiol. 16(1):27-32 (2008).
4. Graham LM, Brown GD. The Dectin-2 family of C-type lectins in immunity and homeostasis. Cytokine, 48(1-2):148-55 (2009).
5. Ritter M, Gross O, Kays S, Ruland J, Nimmerjahn F, et al. Schistosoma mansoni triggers Dectin-2, which activates the Nlrp3 inflammasome and alters adaptive immune responses. Proc Natl Acad Sci U S A. 2010 Nov 8. [Epub ahead of print].
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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