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Monoclonal Antibody to Dectin-2 (Clone IMG3D1) Alexa Fluor® 647 Conjugate |
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| Catalog No: |
IMG-6682AF647 |
| Contents: |
Antibody in 1 ml PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic. |
| Isotype: |
Mouse IgG3, Kappa |
| Clone: |
IMG3D1 |
| Purification: |
Protein G Chromatography |
| Species: |
Chimpanzee, Human, Mouse, Orangutan, Rat |
| Host: |
Mouse |
| Application: |
Flow (Cell Surface): 10 ul/5x10^5 cells (0.5 ug) |
| Storage: |
Store at 4°C, stable for 6 months. DO NOT FREEZE; MATERIAL IS LIGHT-SENSITIVE. |
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Background
Dectin-2 is a C-type lectin expressed by dendritic cells, macrophages and inflammatory monocytes. It is a type II membrane receptor, with a short cytoplasmic tail, and a C-terminal C-type lectin domain. Dectin-2 is a receptor with specificity for high mannose structures and mediates cytokine production from DC and other antigen presenting cells. Though, Dectin-2 has no obvious signaling motifs attached to CP tail, reports indicate that Dectin-2 employs FcRγ for internalization and to activate NF-ķB pathway for cytokine production. Though it shares only 20-25% of homology with Dectin-1, it functions similarly as pattern recognition receptor (PRR) for fungal infections, to activate both innate (Syk-CARD9 innate signaling pathway) and adaptive immune responses (by activation of DC) to fungal infections. Thus Dectin-2 is a connector molecule of both innate and adaptive immunity functioning at DC level. |
Antigen
A portion of amino acids 75-125 of human Dectin-2 was used as the immunogen. |
Application Notes
The amino acids sequence used as immunogen is 100% homologous in human, orangutan and chimp, 83% homologous in rhesus monkey, bovine and sheep, 65% homologous in mouse and 52% homologous in rat. |
Genebank Info (Protein)
NP_001007034.1 |
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Cell surface flow cytometric analysis of Dectin-2 expression by 5x10^5 human peripheral blood monocytes using 10 ul (0.25 ug) of IMG-6350D (anti-CD14), 10 ul (0.5 ug) of IMG-6682AF647 (anti-Dectin-2) and 0.5 ug of isotype control. IMGENEX’s cell surface flow kit (10084K) was used for this test. |
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Cell surface flow cytometric analysis of Dectin-2 expression by 5x10^5 human peripheral blood monocytes using 10 ul (0.5 ug) of IMG-6682AF647 (red) and 0.5 ug of isotype control (green). IMGENEX’s cell surface flow kit (10084K) was used for this test. |
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Reference
1. Flornes LM, Bryceson YT, Spurkland A, Lorentzen JC, Dissen E, Fossum S. Identification of lectin-like receptors expressed by antigen presenting cells and neutrophils and their mapping to a novel gene complex. Immunogenetics, 56(7):506-517 (2004).
2. Kanazawa N, Tashiro K, Inaba K, Lutz MB, Miyachi Y. Molecular cloning of human dectin-2. J Invest Dermatol. 122(6):1522-1524 (2004).
3. Willment JA, Brown GD. C-type lectin receptors in antifungal immunity. Trends Microbiol. 16(1):27-32 (2008).
4. Graham LM, Brown GD. The Dectin-2 family of C-type lectins in immunity and homeostasis. Cytokine, 48(1-2):148-55 (2009).
5. Ritter M, Gross O, Kays S, Ruland J, Nimmerjahn F, et al. Schistosoma mansoni triggers Dectin-2, which activates the Nlrp3 inflammasome and alters adaptive immune responses. Proc Natl Acad Sci U S A. 2010 Nov 8. [Epub ahead of print].
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for six months from the date of purchase.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc. IMGENEX sells Alexa Fluor® dye conjugated antibodies under license from Molecular Probes, Inc. for research use only.
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