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Product : Antibody >> Cytokine & Chemokine , Innate Immunology , New Antibodies , Th17 Antibodies >> EBI3
      
 

Monoclonal Antibody to EBI3 (Clone IMG10J706)

 
Cat. No.DescriptionFormatUnitPrice(USD)QuantityBulk Quotation
IMG-6706A
EBI3Purified
0.1 mg
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Catalog No: IMG-6706A
Imgenex is now a part of Novus Biologicals! All orders, billing and shipments will be received from Novus. Please contact Novus directly for any questions – novus@novusbio.com. To insure proper processing of you order, please insure that your purchase orders show Novus Biologicals as the vendor.
Contents: 100 ug antibody in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Isotype: Rat IgG2b, Kappa
Clone: IMG10J706
Purification: Protein G Chromatography
Species: Mouse
Host: Rat
Application: Flow (Intracellular): 1-3 ug/10^6 cells
Western blot analysis: 0.1-3 ug/ml
Storage: Store antibody at 4°C, stable for 6 months. For long-term storage, aliquot and store at -20°C.
 

Background
EBI3 (Epstein-Barr virus Induced-3) is a secreted glycoprotein of the hematopoietin receptor family. It plays a critical regulatory role in the induction of Th2-type immune responses and the development of Th2-mediated tissue inflammation in vivo, which may be mediated through the control of iNKT cell function. EBI3 dimerizes with p28 and p35 subunits of IL-12 to form new proteins IL-27 and IL-35, respectively (Honglian Tong et al, 2010). IL-27 is an early product of activated antigen presenting cell that is produced upon TLR ligation. It negatively regulates Th17 cell differentiation.  EBI3 is widely expressed and its expression in dendritic cell is transcriptionally regulated by TLR signaling via MyD88 and NF-kappaB during innate immune responses preceding cytokine driven Th cell development. EBI3 signal inhibits delayed-type hypersensitivity responses by suppressing IL-17 production and inducing IL-10 hyperproduction. EBI3 may play a novel role in controlling tumor metastasis via lung CD8+ T cells, and its deficiency is associated with a diminished production of Th2 cytokines which are known to regulate allergic airway inflammation in asthma. Targeted deletion of EBI3 protects mice from lung metastasis.

Antigen
Full-length recombinant mouse EBI3 protein

Application Notes
View additional EBI3 antibodies

Genebank Info (Protein): NP_056581
Gene ID: 50498
SwissProt: O35228
 
EBI3 Antibody

Western blot testing of mouse EBI3 monoclonal antibody at 0.1 ug/ml on recombinant protein. Goat anti-rat Ig HRP secondary antibody (20201) and PicoTect ECL substrate solution (10087K) were used for this test.

EBI3 Antibody IMG-6706A

Figure A: Intracellular flow cytometric analysis of mouse IL-35 in transfected and untransfected HEK 293 cells (Brefeldin A treated, 5 hours) using mouse EBI3 antibody at 3 ug/10^6 cells.

Figure B: Intracellular flow cytometric analysis of mouse EBI3 in stimulated and unstimulated RAW cells (LPS treated, 50 ng/ml, overnight) using mouse EBI3 antibody at 1.5 ug/10^6 cells.

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Reference
1. Tong H, Miyazaki Y, Yamazaki M, Hara H, Waldmann H, Hori S, Yoshida H. Exacerbation of delayed-type hypersensitivity responses in EBV-induced gene-3 (EBI-3)-deficient mice. Immunol Lett. 2010 Feb 16;128(2):108-15. Epub 2010 Jan 12. PMID: 20064562
2. Sauer KA, Maxeiner JH, Karwot R, Scholtes P, Lehr HA, Birkenbach M, Blumberg RS, Finotto S. Immunosurveillance of lung melanoma metastasis in EBI-3-deficient mice mediated by CD8+ T cells. J Immunol. 2008 Nov 1;181(9):6148-57. PMID: 18941205
3. Wirtz S, Becker C, Fantini M, Nieuwenhuis E, Tubbe I, Galle P, Schild H, Birkenbach M, Blumberg R, Neurath M.  EBV-Induced Gene 3 Transcription Is Induced by TLR Signaling in Primary Dendritic Cells via NF-kB Activiation. J Immunol. 2005 174; 2814-2824.


Research purposes only. Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.


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