Product : Antibody >> Cytokine & Chemokine , Innate Immunology , New Antibodies , Th17 Antibodies >> EBI3

Monoclonal Antibody toEBI3 (Clone IMG10J811)

Cat. No.DescriptionFormatUnitPrice(USD)QuantityBulk Quotation
0.1 mg
Catalog No: IMG-6709A
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Contents: 100 ug antibody in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Isotype: Rat IgG2a, Kappa
Clone: IMG10J811
Purification: Protein G Chromatography
Species: Mouse
Host: Rat
Application: Flow (Intracellular): 1-3 ug/ml
Western blot analysis: 0.1-3 ug/ml
Storage: Store antibody at 4C, stable for 6 months. For long-term storage, aliquot and store at -20C.

EBI3 (Epstein-Barr virus Induced-3) is a secreted glycoprotein of the hematopoietin receptor family. It plays a critical regulatory role in the induction of Th2-type immune responses and the development of Th2-mediated tissue inflammation in vivo, which may be mediated through the control of iNKT cell function. EBI3 dimerizes with p28 and p35 subunits of IL-12 to form new proteins IL-27 and IL-35, respectively (Honglian Tong et al, 2010). IL-27 is an early product of activated antigen presenting cell that is produced upon TLR ligation. It negatively regulates Th17 cell differentiation. EBI3 is widely expressed and its expression in dendritic cell is transcriptionally regulated by TLR signaling via MyD88 and NF-kappaB during innate immune responses preceding cytokine driven Th cell development. EBI3 signal inhibits delayed-type hypersensitivity responses by suppressing IL-17 production and inducing IL-10 hyperproduction. EBI3 may play a novel role in controlling tumor metastasis via lung CD8+ T cells, and its deficiency is associated with a diminished production of Th2 cytokines which are known to regulate allergic airway inflammation in asthma. Targeted deletion of EBI3 protects mice from lung metastasis (Kerstin A et al, 2008).

Full-length recombinant mouse EBI3 protein

Application Notes
View additional EBI3 antibodies

Genebank Info (Protein): NP_056581
Gene ID: 50498
SwissProt: O35228
EBI3 Antibody

Western blot testing of mouse EBI3 monoclonal antibody at A) 0.1 ug/ml on recombinant protein and B) 3 ug/ml on recombinant mouse IL-35 protein (IMR-400-1) Goat anti-rat Ig HRP secondary antibody (20201) and PicoTect ECL substrate solution (10087K) were used for this test.

EBI3 Antibody IMG-6709A

Figure A: Intracellular flow cytometric analysis of mouse IL-35 in transfected and untransfected HEK 293 cells (Brefeldin A treated, 5 hours) using mouse EBI3 antibody at 3 ug/10^6 cells.

Figure B: Intracellular flow cytometric analysis of mouse EBI3 in stimulated and unstimulated RAW cells (LPS treated, 50 ng/ml, overnight) using mouse EBI3 antibody at 1.5 ug/10^6 cells.


1. Tong H, Miyazaki Y, Yamazaki M, Hara H, Waldmann H, Hori S, Yoshida H. Exacerbation of delayed-type hypersensitivity responses in EBV-induced gene-3 (EBI-3)-deficient mice. Immunol Lett. 2010 Feb 16;128(2):108-15. Epub 2010 Jan 12. PMID: 20064562
2. Sauer KA, Maxeiner JH, Karwot R, Scholtes P, Lehr HA, Birkenbach M, Blumberg RS, Finotto S. Immunosurveillance of lung melanoma metastasis in EBI-3-deficient mice mediated by CD8+ T cells. J Immunol. 2008 Nov 1;181(9):6148-57. PMID: 18941205
3. Wirtz S, Becker C, Fantini M, Nieuwenhuis E, Tubbe I, Galle P, Schild H, Birkenbach M, Blumberg R, Neurath M.  EBV-Induced Gene 3 Transcription Is Induced by TLR Signaling in Primary Dendritic Cells via NF-kB Activiation. J Immunol. 2005 174; 2814-2824.

Research purposes only. Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.






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