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EBI3 Antibody Sampler Kit |
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| Catalog No: |
IMG-6712k |
| Contents: |
25 ug per sample |
| Isotype: |
Please see individual datasheets |
| Purification: |
Protein A or G Chromatography |
| Species: |
Human, Mouse, New World monkey, Rat |
| Host: |
Multiple |
| Application: |
Western blot analysis, Flow (Intracellular), IHC (please see individual datasheets for details) |
| Storage: |
Store at 4°C, stable for 6 months. For long-term storage, aliquot and store at -20°C. |
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Background
EBI3 (Epstein-Barr virus Induced-3) is a secreted glycoprotein of the hematopoietin receptor family. It plays a critical regulatory role in the induction of Th2-type immune responses and the development of Th2-mediated tissue inflammation in vivo, which may be mediated through the control of iNKT cell function. EBI3 dimerizes with p28 and p35 subunits of IL-12 to form new proteins IL-27 and IL-35, respectively (Honglian Tong et al, 2010). IL-27 is an early product of activated antigen presenting cell that is produced upon TLR ligation. It negatively regulates Th17 cell differentiation. EBI3 is widely expressed and its expression in dendritic cell is transcriptionally regulated by TLR signaling via MyD88 and NF-kappaB during innate immune responses preceding cytokine driven Th cell development. EBI3 signal inhibits delayed-type hypersensitivity responses by suppressing IL-17 production and inducing IL-10 hyperproduction. EBI3 may play a novel role in controlling tumor metastasis via lung CD8+ T cells, and its deficiency is associated with a diminished production of Th2 cytokines which are known to regulate allergic airway inflammation in asthma. Targeted deletion of EBI3 protects mice from lung metastasis (Kerstin A et al, 2008). Following are the different antibodies included in this EBI3 Sampler Kit:
| Description |
Cat. No. |
Qty |
Application |
Clonality |
Species Reactivity |
Format |
| EBI3 |
IMG-6100A |
25 ug |
WB |
pAb |
H, Mk |
Purified |
| EBI3 |
IMG-6185A |
25 ug |
WB |
pAb |
H, M |
Purified |
| EBI3 |
IMG-6327A |
25 ug |
WB, IHC |
pAb |
M, R |
Purified |
| EBI3 |
IMG-6706A |
25 ug |
WB, Flow |
mAb |
M |
Purified |
| EBI3 |
IMG-6707A |
25 ug |
WB, Flow |
mAb |
M |
Purified |
| EBI3 |
IMG-6708A |
25 ug |
WB, Flow |
mAb |
M |
Purified |
| EBI3 |
IMG-6709A |
25 ug |
WB, Flow |
mAb |
M |
Purified |
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WB: Western Blot IHC: Immunohistochemistry Flow: Flow Cytometry H: Human M: Mouse Mk: Monkey R: Rat | |
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IMG-6709A
Western blot testing of mouse EBI3 monoclonal antibody at A) 0.1 ug/ml on recombinant protein and B) 3 ug/ml on recombinant mouse IL-35 protein (IMR-400-1) Goat anti-rat Ig HRP secondary antibody (20201) and PicoTect ECL substrate solution (10087K) were used for this test. |
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IMG-6708A
Figure A: Intracellular flow cytometric analysis of mouse IL-35 in transfected and untransfected HEK 293 cells (Brefeldin A treated, 5 hours) using anti-mouse EBI3 antibody at 3 ug/10^6 cells.
Figure B: Intracellular flow cytometric analysis of mouse EBI3 in stimulated and unstimulated RAW cells (LPS treated, 50 ng/ml, overnight) using anti-mouse EBI3 antibody at 1.5 ug/10^6 cells. |
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IMG-6327A
Immunohistochemical analysis of EBI3 in formalin-fixed, paraffin-embedded mouse brain tissue using an isotype control (top left) and anti-EBI3 antibody (bottom left, right) at 5 ug/ml. |
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Reference
1. Tong H, Miyazaki Y, Yamazaki M, Hara H, Waldmann H, Hori S, Yoshida H. Exacerbation of delayed-type hypersensitivity responses in EBV-induced gene-3 (EBI-3)-deficient mice. Immunol Lett. 2010 Feb 16;128(2):108-15. Epub 2010 Jan 12. PMID: 20064562
2. Sauer KA, Maxeiner JH, Karwot R, Scholtes P, Lehr HA, Birkenbach M, Blumberg RS, Finotto S. Immunosurveillance of lung melanoma metastasis in EBI-3-deficient mice mediated by CD8+ T cells. J Immunol. 2008 Nov 1;181(9):6148-57. PMID: 18941205
3. Wirtz S, Becker C, Fantini M, Nieuwenhuis E, Tubbe I, Galle P, Schild H, Birkenbach M, Blumberg R, Neurath M. EBV-Induced Gene 3 Transcription Is Induced by TLR Signaling in Primary Dendritic Cells via NF-kB Activiation. J Immunol. 2005 174; 2814-2824.
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Research purposes only.
Not for diagnostic or in vivo use.
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