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Polyclonal Antibody to Cactin |
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| Catalog No: |
IMG-6714A |
| Contents: |
100 µg in 200 µl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic. |
| Isotype: |
Rabbit IgG |
| Purification: |
Protein A Chromatography |
| Species: |
Chimpanzee, Cow, Dog, Human, Opossum, Rhesus Monkey, Wild pig |
| Predicted React: |
bacteria, Mosquito, Mouse, Pufferfish, Rat, wild turkey, Zebrafish |
| Host: |
Rabbit |
| Application: |
IHC (paraffin): 5 ug/ml
Western blot analysis: 5-7 ug/ml |
| Storage: |
Store at 4°C, stable for 6 months. For long term storage, aliquot and store at -20°C. |
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Background
Cactin is a novel negative regulator of TLR signaling and reveals its capacity to target MHC Class III genes at the molecular and functional level. It is localized to the nucleus, which is critical for manifesting its inhibitory effects on TLR signaling. Experimental evidences show overexpression of Cactin suppresses TLR-induced activation of NF-kB and interferon-regulatory factor transcription factors and induction of TLR-responsive genes. It is also reported Cactin interacts with IkB-like protein and targets other proteins that are encoded by genes in the MHC Class III region of chromosome 6. |
Antigen
A portion of amino acid 460-510 of human Cactin was used as the immunogen. |
Application Notes
The amino acid sequence used as immunogen is 100% homologous in human, chimpanzee, rhesus monkey, 88% homologous in wild pig, 82% homologous in dog, cow, opossum, 64% homologous in mouse, rat, wild turkey, mosquito and 47% homologous in zebrafish, pufferfish, and bacteria. |
Genebank Info (Protein)
NP_067054 |
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Western blot analysis of Cactin. Lanes 1 and 2 show human heart tissue lysate probed with pre-immune sera (1:5000) and Cactin polyclonal antibody (7 ug/ml), respectively. |
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IHC staining of human brain using Cactin polyclonal antibody at 5 ug/ml. |
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Reference
1. Atzei, P. et al. J. Biol. Chem. 285: 36804-36817 (2010).
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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