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Polyclonal Antibody to AP3 Delta |
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| Catalog No: |
IMG-6730A |
| Contents: |
100 ug antibody antibody in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic. |
| Isotype: |
Rabbit IgG |
| Purification: |
Protein A Chromatography was used to purify the AP3 antibody |
| Species: |
Chimpanzee, Human, New World monkey, Orangutan, Rhesus Monkey |
| Predicted React: |
Cow, Dog, Horse, Mouse, Opossum, Panda, Rat |
| Host: |
Rabbit |
| Application: |
IHC (p): 5 ug/ml
Western blot analysis: 2-5 ug/ml |
| Storage: |
Store AP3 antibody at 4°C, stable for 6 months. For long-term storage, aliquot and store antibody at -20°C. |
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Background
AP3 delta (AP3D1) is part of the AP3 complex, an adaptor-related complex which is not clathrin-associated. The complex is associated with the Golgi region as well as more peripheral structures. It facilitates the budding of vesicles from the Golgi membrane and may be directly involved in trafficking to lysosomes. Adaptor protein complex 3 (AP3) is a heterotetramer composed of two large adaptins (delta-type subunit AP3D1 and beta- type subunit AP3B1 or AP3B2), a medium adaptin (mu-type subunit AP3M1 or AP3M2) and a small adaptin (sigma-type subunit APS1 or AP3S2). The AP3 Delta subunit is implicated in intracellular biogenesis and trafficking of pigment granules and possibly platelet dense granules and neurotransmitter vesicles. Five isoforms of the human protein are produced by alternative splicing. |
Antigen
A portion of amino acids 530-580 of human AP3 Delta was used as the immunogen for the antibody. |
Application Notes
The AP3 amino acid sequence used as immunogen for this antibody is 100% homologous in human, chimpanzee, orangutan, new world monkey, rhesus monkey, and 63% homologous in panda, carolina anole, opossum, and 58% homologous in taipan, zebra finch, horse, dog, pig and 53% homologous in cow, rat, mouse. |
Genebank Info (Protein)
NP_001070991.1 |
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Western blot analysis using AP3 Delta antibody. HeLa lysate in the 1) absence and 2) presence of immunizing peptide, probed with AP3 Delta antibody at 2 ug/ml. IMGENEX's goat anti-rabbit Ig HRP secondary antibody (20301) and PicoTect ECL substrate solution (10087K) were used for this test. |
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IHC of human rectum tissue probed with AP3 Delta antibody at 5 ug/ml. |
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Reference
1. Miwa, S. et al. SCIENCE 329: 1530-134 (2010)
2. Hashimoto, R. et al. Neurosci. Res. 65: 113-115 (2009)
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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