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Monoclonal Antibody to HMGB1 (Clone IMG19N15F4) Alexa Fluor® 647 Conjugate |
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| Catalog No: |
IMG-6739AF647 |
| Contents: |
HMGB1 antibody in PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic. |
| Isotype: |
Mouse IgG1, Kappa |
| Clone: |
IMG19N15F4 |
| Purification: |
Protein G Chromatography |
| Species: |
Human, Mouse |
| Host: |
Mouse |
| Application: |
Flow (Intracellular): 10 ul/test/5x10^5 cells (1 ug) |
| Storage: |
Store antibody at 4°C, stable for 6 months. DO NOT FREEZE; MATERIAL IS LIGHT-SENSITIVE. |
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Background
HMGB1 (high mobility group protein B1) belongs to the HMGB family and contains two HMG box DNA-binding domains. It is a highly conserved, ubiquitous protein present in the nuclei and cytoplasm of nearly all cell types, and is a necessary and sufficient mediator of inflammation during sterile and infection-associated responses. HMGB1 also act as DNA nuclear binding protein that has recently been shown to be an early trigger of sterile inflammation in animal models of trauma-hemorrhage via the activation of the Toll-like receptor 4 (TLR4) and the receptor for the advanced glycation endproducts (RAGE). It is reported that the level of HMGB1 is elevated during sterile tissue injury, infection, lethal endotoxemia or sepsis, collagen-induced arthritis, and ischemia-reperfusion induced tissue injury. |
Antigen
Full-length recombinant human HMGB1 was used as the immunogen. |
Application Notes
This antibody recognizes both A box domain (amino acids 9-79) and full length HMGB1 protein. |
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Intracellular flow cytometric analysis using HMGB1 antibody. Human Jurkat cells were probed using 10 ul of HMGB1 antibody (red) and 1 ug of isotype control antibody (20109AF647, green). |
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Reference
1. Yang, H. et al. Biochim. Biophys. Acta. 1799:149-156 (2010).
2. Cohen, MJ. et al. Crit. Care. 13:R174 (2009).
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for six months from the date of purchase.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc. IMGENEX sells Alexa Fluor® dye conjugated antibodies under license from Molecular Probes, Inc. for research use only.
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