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Monoclonal Antibody to HMGB1 (Clone IMG19N12A1) |
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| Catalog No: |
IMG-6740A |
| Contents: |
100 ug antibody in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic. |
| Isotype: |
Mouse IgG2b, Kappa |
| Clone: |
IMG19N12A1 |
| Purification: |
Protein G Chromatography |
| Species: |
Human, Mouse |
| Host: |
Mouse |
| Application: |
Western blot analysis: 1-3 ug/ml |
| Storage: |
Store antibody at 4°C, stable for 6 months. For long-term storage, aliquot and store at -20°C. |
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Background
HMGB1 (high mobility group protein B1) belongs to the HMGB family and contains two HMG box DNA-binding domains. It is a highly conserved, ubiquitous protein present in the nuclei and cytoplasm of nearly all cell types, and is a necessary and sufficient mediator of inflammation during sterile and infection-associated responses. HMGB1 also act as DNA nuclear binding protein that has recently been shown to be an early trigger of sterile inflammation in animal models of trauma-hemorrhage via the activation of the Toll-like receptor 4 (TLR4) and the receptor for the advanced glycation endproducts (RAGE). It is reported that the level of HMGB1 is elevated during sterile tissue injury, infection, lethal endotoxemia or sepsis, collagen-induced arthritis, and ischemia-reperfusion induced tissue injury. |
Antigen
Full-length recombinant human HMGB1 was used as the immunogen. |
Application Notes
This antibody recognizes bothB box domain (amino acids 88-162) and full length HMGB1 protein. |
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Western blot analysis using HMGB1 antibody. A) Full-length human HMGB1 protein, B) human HeLa cell lysate and C) mouse NIH 3T3 cell lysate were probed with HMGB1 antibody at 2 ug/ml. IMGENEX's goat anti-mouse Ig HRP secondary antibody (20101) and PicoTect ECL substrate solution (10087K) were used for this test. |
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Reference
1. Yang, H. et al. Biochim. Biophys. Acta. 1799:149-156 (2010).
2. Cohen, MJ. et al. Crit. Care. 13:R174 (2009).
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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