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Monoclonal Antibody to human Podoplanin (Clone NZ-1.2) |
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| Catalog No: |
IMG-6742AF647 |
| Contents: |
Podoplanin antibody in PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic. |
| Isotype: |
Rat IgG2a, Lambda |
| Clone: |
NZ-1.2 |
| Purification: |
Protein G Chromatography |
| Species: |
Human |
| Host: |
Rat |
| Application: |
Flow (Cell Surface): 10 ul/test/10^6 cells (0.15 ug) |
| Storage: |
Store antibody at 4°C, stable for 6 months. DO NOT FREEZE; MATERIAL IS LIGHT-SENSITIVE. |
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Background
Podoplanin is a mucin type-1 glycoprotein of 40-43kd also know as T1A, gp38 or Aggrus. Podoplanin is expressed in many tumors and many normal cells, especially lymphatic epithelial cells and follicular DCs. It appears to serve as a ligand for CLEC-2. Podoplanin expression is positively correlated with tumors expressing greater invasive and metastatic potential. Podoplanin is directly involved in cell migration, aids metastases formation and tumor cell invasion of tissue (Schacht, 2005, Raica, 2008, Martin-Villar, 2010). It has also been determined that podoplanin expression is induced through Src activation of Cas (Shen, 2010). Recently there are indications of podoplanin presence on Th17 cells (Bettelli, 2011). Initial studies below indicate the presence of podoplanin on a small but distinct population of CD4+ cells from human PBMC. |
Antigen
A synthetic peptide corresponding to amino acids 38-51 (EGGVAMPGAEDDVV) from the platelet-aggregation-stimulating (PLAG) domain of human podoplanin was used as immunogen, Gene Bank no sp|Q86YL7.3|PDPN_HUMAN. |
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Flow cytometric analysis using Podoplanin antibody. Human NCCIT cells were probed using 10 ul (0.15 ug) of Podoplanin antibody (red) and 0.15 ug of isotype control antibody (green). IMGENEX’s flow kit (10084K) was used for this test. |
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Flow cytometric analysis using Podoplanin antibody. Human PBMC were probed using 10 ul of CD4 antibody and 10 ul or 0.15 ug of isotype control antibody or Podoplanin antibody. IMGENEX’s flow kit (10084K) was used for this test. |
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Reference
1. Schacht, V et al. 2005 Am. J. Pathol. 166: 913
2. Raica, M et al. 2008 Anticancer Research 28: 2997
3. Martin-Villar , E et al 2010 Mol. Bio.Cell 21: 4387.
4. Shen, Y et al 2010 JBC 285:9649
5. Bettelli, e 2011 http://projectreporter.nih.gov/project_info_description.cfm?icde=0&aid=7899876 Project Number 5RO1NS0599996-04
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for six months from the date of purchase.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc. IMGENEX sells Alexa Fluor® dye conjugated antibodies under license from Molecular Probes, Inc. for research use only.
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