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Product : Antibody >> Alexa Fluor Antibodies , Innate Immunology , Th17 Antibodies >> Podoplanin
      
 

Monoclonal Antibody to mouse Podoplanin (Clone pmab-1) Alexa Fluor® 647 Conjugate

 
Cat. No.DescriptionFormatUnitPrice(USD)QuantityBulk Quotation
IMG-6743AF647-25
IMG-6743AF647-100
PodoplaninAlexa Fluor® 647
25 µg
100 µg
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Catalog No: IMG-6743AF647-25
Imgenex is now a part of Novus Biologicals! All orders, billing and shipments will be received from Novus. Please contact Novus directly for any questions – novus@novusbio.com. To insure proper processing of you order, please insure that your purchase orders show Novus Biologicals as the vendor.
Contents: Podoplanin antibody in PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Isotype: Rat IgG2a, Kappa
Clone: pmab-1
Purification: Protein G Chromatography
Species: Mouse
Host: Rat
Application: Flow (Cell Surface): 0.1-0.5 ug/10^6 cells
Storage: Store antibody at 4°C, stable for 6 months. DO NOT FREEZE; MATERIAL IS LIGHT-SENSITIVE.
 

Background
Podoplanin, also known as gp38, is a 43kD type 1 transmembrane glycoprotein found on many tissues. Podoplanin appears to be differentially expressed by lymphatic endothelial cells but not blood vessel endothelial cells (Schacht, 2005). Podoplanin has also been found to be expressed on a wide variety of tumors. It appears to be involved in lymphangioigenesis and promoting cell migration (Farr, 1992).

Recently, there are indications of podoplanin presence on Th17 cells (Bettelli, 2011). In a murine EAE model for autoimmune disease it has been shown that Th17 cells migrate quickly to the CNS at EAE onset. Injection of antibody to podoplanin in mice also immunized with myelin antigen to induce EAE results in elevated numbers of Th17 cells in the CNS. Thus as in effecting migration of tumor cells, podoplanin may also promote migration of committed Th17 cells to sites of inflammation in autoimmune diseases.

 
Podoplanin Antibody

Flow cytometric analysis using podoplanin antibody. Mouse LLC1 cells were probed using 0.5 ug of podoplanin antibody (red) and 0.5 ug of isotype control antibody (green). IMGENEX’s cell surface flow kit (10084K) was used for this test.

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Reference
1. Schacht, V et al. 2005 Am. J. Pathol. 166: 913
2. Bettelli, e 2011 http://projectreporter.nih.gov/project_info_description.cfm?icde=0&aid=7899876  Project Number 5RO1NS0599996-04
3. Farr, A et al. 1992 J. Histochem Cytochem 40:651


Research purposes only. Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for six months from the date of purchase.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc. IMGENEX sells Alexa Fluor® dye conjugated antibodies under license from Molecular Probes, Inc. for research use only.

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