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Product : Antibody >> Innate Immunology , Th17 Antibodies >> Podoplanin
      
 

Monoclonal Antibody to mouse Podoplanin (Clone pmab-1) Azdie-Free

 
Cat. No.DescriptionFormatUnitPrice(USD)QuantityBulk Quotation
IMG-6743E-50
PodoplaninAzide-Free
50 µg
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Catalog No: IMG-6743E-50
Imgenex is now a part of Novus Biologicals! All orders, billing and shipments will be received from Novus. Please contact Novus directly for any questions – novus@novusbio.com. To insure proper processing of you order, please insure that your purchase orders show Novus Biologicals as the vendor.
Contents: Podoplanin antibody in PBS. Filter sterilized. Sodium azide free.
Isotype: Rat IgG2a, Kappa
Clone: pmab-1
Purification: Protein G Chromatography
Species: Mouse
Host: Rat
Application:
Western blot analysis: 3-5 ug/ml
Storage: Aliquot antibody and store frozen at -20°C.
 

Background
Podoplanin, also known as gp38, is a 43kd type 1 transmembrane glycoprotein found on many tissues. Podoplanin appears to be differentially expressed by lymphatic endothelial cells but not blood vessel endothelial cells (Schacht, 2005). Podoplanin has also been found to be expressed on a wide variety of tumors. It appears to be involved in lymphangioigenesis and promoting cell migration (Farr, 1992).

Recently, there are indications of podoplanin presence on Th17 cells (Bettelli, 2011). In a murine EAE model for autoimmune disease it has been shown that Th17 cells migrate quickly to the CNS at EAE onset. Injection of antibody to podoplanin in mice also immunized with myelin antigen to induce EAE results in elevated numbers of Th17 cells in the CNS. Thus as in effecting migration of tumor cells, podoplanin may also promote migration of committed Th17 cells to sites of inflammation in autoimmune diseases.

Antigen
A synthetic peptide corresponding to amino acids 38-51 (EGGVAMPGAEDDVV) from the platelet-aggregation-stimulating (PLAG) domain of human podoplanin was used as immunogen, Gene Bank no sp|Q86YL7.3|PDPN_HUMAN.

 
Podoplanin Antibody

Western blot analysis using podoplanin antibody. Lysate from mouse A) Brain, B) Heart, C) Small Intestine, D) Kidney, E) Liver, F) Lung, G) Muscle, H) Stomach, I) Spleen, J) Ovary, and K) Testis probed with podoplanin antibody at 5 ug/ml. IMGENEX's mouse tissue Insta-blot (IMB-101), goat anti-rat Ig HRP secondary antibody (20201) and PicoTect ECL substrate solution (10087K) were used for this test.

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Reference
1. Schacht, V et al. 2005 Am. J. Pathol. 166: 913
2. Bettelli, e 2011 http://projectreporter.nih.gov/project_info_description.cfm?icde=0&aid=7899876  Project Number 5RO1NS0599996-04
3. Farr, A et al. 1992 J. Histochem Cytochem 40:651


Research purposes only. Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.


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