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Polyclonal Antibody to GBP1 |
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| Catalog No: |
IMG-6752A |
| Contents: |
100 ug antibody in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic. |
| Isotype: |
Rabbit IgG |
| Purification: |
Protein A Chromatography |
| Species: |
Human, Mouse |
| Predicted React: |
Rat |
| Host: |
Rabbit |
| Application: |
IHC (paraffin): 5 ug/ml
Western blot analysis: 2-4 ug/ml |
| Storage: |
Store antibody at 4°C, stable for 6 months. For long-term storage, aliquot and store antibody at -20°C. |
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Background
GBP1 or guanlyate binding protein 1 is a 68 kDa GTPase and member of the GTPase1 family of molecules (Kunzelman, 2006). GBP1 expression is induced by Interferon g as well as other inflammatory cytokines including IL-1a, IL-1b and TNF-a (Naschberger, 2004). Induction of GBP1 expression by poly I:C, a prototypical TLR3 ligand, as well as GBP1 expression dependency on NF-kB and IFNa response elements implicate TLR pathway involvement (Naschberger, 2004). Inversely proportional expression of GBP1 and MMP-1 as well as other related molecules to adhesion and migration show a clear functional role of GBP1 in suppressing angiogenesis and migration especially of endothelial cells (Guenzi, 2003). As a regulatory molecule it is centrally involved in signal transduction of inflammatory and cell migration networks. |
Antigen
A portion of amino acids 500-550 of human GBP1 was used as the immunogen for this antibody. |
Application Notes
The amino acid sequence used as immunogen is 100% homologous in human, 95% in rat and 79% in mouse. |
Genebank Info (Protein)
NP_002044.2 |
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Western blot analysis of GBP1 using GBP1 polyclonal antibody. Human HCT-116 lysate in the absence (1) and presence (2) of immunizing peptide and mouse RAW cell lysate (3) probed with GBP1 antibody at 3 ug/ml. IMGENEX’s goat anti-rabbit Ig HRP secondary antibody (20301) and PicoTect ECL substrate solution (10087K) were used for this test. |
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Immunohistochemical analysis of GBP1 using GBP1 polyclonal antibody. Human rectum tissue probed with GBP1 antibody at 5 ug/ml. |
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Reference
1. Kunzelman, S et al 2006 J.Biol. chem.. 281: 28627-28635
2. Naschberger, E et al 2004 Biochem J.379: 409-420
3. Guenzi, E et al 2003 EMBO J. 22: 3772-3782
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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