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Polyclonal Antibody to DDB1 |
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| Catalog No: |
IMG-6799A |
| Contents: |
0.1 mg antibody in 0.2 ml PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic. |
| Isotype: |
Rabbit IgG |
| Purification: |
Protein A Chromatography |
| Species: |
Cat, Chimpanzee, Cow, Dog, Elephant, Horse, Human, Macaque, Monkey, Mouse, Orangutan, Panda, Pig, Rat, Rhesus Monkey |
| Predicted React: |
Chicken, Chinese Hamster, Frog, Guinea Pig |
| Host: |
Rabbit |
| Application: |
Flow (Intracellular): 0.2 ug/10^6 cells
Western blot analysis: 2-4 ug/ml
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| Storage: |
Store DDB1 antibody at 4°C, stable for 6 months. For long-term storage, aliquot and store antibody at -20°C. |
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Background
DNA Damage Binding protein 1 (DDB1) binds with CUL4 to form an ubiquitin E3 ligase that targets protein substrates for ubiquitin-dependent proteolysis (1, 2). Loss of DDB1 impedes the proteolysis of a grouping of CUL4 substrates at least in response to UV (3, 4) and CUL4 ubiquitinylation (5). DDB1 is highly conserved from yeast to mammals (3, 6). DDB1 specific antibody probes might be useful in identifying CUL4-DDB1 substrates in epigenetics applications and ubiquitin modification dependent protein function. |
Antigen
A portion of amino acids 385-435 from human DDB1 was used as the immunogen for this antibody. |
Application Notes
The amino acid sequence used as immunogen for this antibody is 100% homologous in human, panda, cow, dog, old world monkey, horse, african bush elephant, crab-eating macaque, rhesus monkey, mouse, chimpanzee, orangutan, rat, pig, and european pole-cat , 94% in guinea pig, chinese hamster, platypus, and zebra finch, 89% in carolina anole, zebrafish, mole rat, cichlid fish, and salmon, 83% in chicken, western clawed frog, and african clawed frog, 78% in wild turkey, and 61% homolgous in common grape vine, soya bean, and tomato. |
| Genebank Info (Protein): NP_001914.3 |
| Gene ID: 1642 |
| SwissProt: Q16531 |
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Western blot analysis of DDB1 using DDB1 antibody. Human liver tissue lysate in the 1) absence and 2) presence of immunizing peptide, 3) Hela and 4) Daudi lysate probed with 2 ug/ml of DDB1 antibody. IMGENEX’s goat anti-rabbit Ig HRP secondary antibody (20301) and PicoTect ECL substrate solution (10087K) were used for this test. |
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Intracellular flow cytometric analysis of DDB1 in human HeLa and mouse RAW cells using DDB1 antibody at 0.2 ug/10^6 cells. Red represents cells with DDB1 antibody, green represents isotype control antibody (IMGENEX, 20304). IMGENEX's goat anti-rabbit PE secondary antibody (20303) was used. |
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Reference
1. Higa, LA and H Zhang 2007. Stealing the spotlight: CUL4-DDB1 ubiquitin ligase docks WD40-repeat proteins to destroy Cell Division 2
2. Higa, LA 2006. CUL4-DDB1 ubiquitin ligase interacts with multiple WD40-repeat proteins and regulates histone methylation. Nat. Cell. Biol. 8: 1277-1283.
3. Cang Y et al 2006. Deletion of DDB1 in mouse brain and lens leads to p53-dependent elimination of proliferating cells. Cell 127:929-940.
4. Higa LA 2006. Involvement of CUL4 ubiquitin E3 ligases in regulating CDK inhibitors Dacapo/p27Kip1 and cyclin E degradation. Cell Cycle 2006, 5:71-77.
5. Hu J et al 2004. Targeted ubiquitination of CDT1 by the DDB1-CUL4A-ROC1 ligase in response to DNA damage. Nat Cell Biol 6:1003-1009.
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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