Background
p53 has a well established role in the blocking the proliferative action of damaged cells and acting in essence as an anticancer agent. It has been called the guardian of the genome. Phosphorylation of Ser392 in p53 is associated with formation of human tumors. In addition p53 has also been linked to affects of aging and oxidative stress. An increase in p53 has also been linked to deficits in LTP and learning and memory. We raised this polyclonal antibody against a peptide representing the sequence around Ser392 of p53.

Antigen
This antibody was developed against synthetic phosphopeptide corresponding to amino acids residues surrounding the phospho-Ser392 of p53. The antibody was purified by sequential chromatography on phospho- and non-phosphopeptide affinity columns.

Application Notes
In PMA treated NIH 3T3, a ~53 kDa band is observed. The labeling by the antibody was specifically blocked by the Ser392 phosphopeptide used as antigen. The corresponding non-phosphopeptide did not block the immunolabeling. Human and some non-human primates have 100% amino acid sequence identity with the antigen used to raise the antibody. Antibody dilutions and tissue load should be based on tissue type andexpected phosphorylation state expected expression level.

Genebank Info (Protein)
NP_001119584

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Reference
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