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Datasheet
Monoclonal Antibody to Human Progesterone Receptor (Phospho-Ser 294)
Cat.No IMG-682
Description Progesterone Receptor (Ser294)
Format Purified
Unit 0.1 mL
Price(USD) 310.00
MSDS
Catalog No
:
IMG-682
Contents
:
0.1 ml in 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% glycerol. Adequate amount of material to conduct 10 mini Western Blots.
Isotype
:
Mouse-IgG1
Clone
:
608
Purification
:
Protein G Chromatography
Species
:
Human
Host
:
Mouse
Application
IHC: 1:1000-1:2000 Western blot analysis: 1:1000-1:2000 Storage For long-term storage –80°C is recommended, but short-term storage at –20°C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Recommended Positive Control : T47D treated with synthetic progestin agonist R5020 (500 nM)
Background There is accumulating evidence to suggest that progesterone plays an essential role in the regulation of growth and differentiation of mammary glands and thus may play a key role in breast cancer. The biological response to progesterone is mediated by two distinct forms of the human progesterone receptor (A and B forms). In most cell contexts, the B form functions as a transcriptional activator, whereas the A form functions as a transcriptional inhibitor of steroid hormones. Recently it has been demonstrated that there is differential hormone dependent regulation of the phosphorylation of the A and B forms of the receptor. Treatment of T47D breast cancer cells with progestin agonist increases the phosphorylation of Ser190 and Ser294 with different kinetics. These phosphorylation events may differentially affect the transcriptional activity of the receptor.
Antigen
Synthetic phosphopeptide corresponding to amino acids residues surrounding the Phospho-Ser294 of human progesterone receptor. Ammonium sulfate precipitation followed by Protein G affinity purification.
Application Notes
An ~90 kDa band (PR-A isoform) and an ~120 kDa band (PR-B isoform) can be observed in T47D treated with sythetic progestin agonist R5020 (500 nM). The labeling by the antibody was specifically blocked by the phosphopeptide used as antigen. The corresponding non- phosphopeptide did not block the immunolabeling. Antibody dilutions and tissue load should be based on tissue type and expected phosphorylation state expected expression level.
Western Blot of T47D cell lysate specific for the human progesterone receptor phosphorylated at Ser294. As shown in Clemm, et al. (2000), cited below, the antibody specifically reacts with the progesterone receptor phosphorylated at Ser294 in T47D cells.
Reference 1. Clemm, D.L., Sherman, L., Boonyaratanakornkit, V., Schrader, W.T., Weigel, N.L., and Edwards, D.P.,Mol. Endocrinol., 14 (2000) 52 - 65. 2. Lin, V. C. L., Woon, C. T., Aw, S. E., Guo, C.,Endocrinology 144 (2003) 5650 -5657.
Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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