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Polyclonal Antibody to HMG14 |
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| Catalog No: |
IMG-6837A |
| Contents: |
0.1 mg antibody in 0.2 ml PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic. |
| Isotype: |
Rabbit IgG |
| Purification: |
Protein A Chromatography was used to purify this HMG14 antibody |
| Species: |
Cow, Dog, Human, New World monkey, Pig, Rabbit, Rhesus Monkey, White-cheeked gibbon |
| Predicted React: |
Mouse, Elephant, Chimpanzee, Chicken, Guinea Pig, Mole Rat, Opossum, Carolina anole |
| Host: |
Rabbit |
| Application: |
Western blot analysis: 2 ug/ml |
| Storage: |
Store HMG14 antibody at 4°C, stable for 6 months. For long-term storage, aliquot and store antibody at -20°C. |
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Background
HMG14, also known as HMGN1, along with HMG17 and HMGN2 are non-histone proteins that bind nucleosomal cores and nucleosomal DNA (1, 2). Like transcription regulatory factors, they may travel to and from the nucleus or at least dissociate and bind again to DNA promoting the folding and unfolding of DNA regulatory sequences exposed to or hiding from binding sites of regulatory factors (3). Both HMG14 and HMG17 affect remodeling and therefore regulate gene activation or repression through transcription factor activity or DNA repair mechanisms (4, 5). Antibody to HMG14 aids in identifying its role in epigenetic related studies. |
Antigen
A portion of amino acids 1-50 from human HMG14 was used as the immunogen for this antibody. |
Application Notes
The HMG14 amino acid sequence used as immunogen for this antibody is 100% homologous in human, cow, new world monkey, dog, rhesus monkey, white-cheeked gibbon, rabbit, and pig, 94% in guinea pig, mole rat, elephant, chimpanzee, and orangutan, 83% in panda, opossum, mouse, and rat, 78% in zebra finch, 72% in carolina anole, and wild turkey, and 61% homologouus in platypus. |
Genebank Info (Protein)
NP_004956.5 |
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Western blot analysis of HMG14 in Daudi lysate in the 1) absence and 2) presence of immunizing peptide probed with 2 ug/ml of HMG14 antibody. IMGENEX’s goat anti-rabbit Ig HRP secondary antibody (20301) and PicoTect ECL substrate solution (10087K) were used for this test. |
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Reference
1. Hick, R, U Scheer and M Bustin 1998 HMG14 and HMG17 are released form mitotic chromosomes and imported into the nucleus by active transport J. Cell Biol. 143: 1427-1436
2. Rattner, BP, T Yusufzai, and JT Kadonaga 2009 HMGN Proteins act in opposition to ATP-dependent chromatin remodeling factors to restrict nucleosome mobility. Mol. Cell 34:620-626.
3. Trieschmann, L, PJ Alfonso, MP Crippa, AP Wolffe, and M Bustin 1998 Incorporation of chromosomal proteins HMG14/HMG17. Into nascent nucleosomes induces an extended chromatin conformation and enhances the utilization of active transcription complexes EMBO J 14: 1478-1489.
4. Birger, Y, KL West, YV Postnikov, JH Lin, T Furusawas, JLK Wagner, CS Laufer, KN Kraemer and M Bustin 2003 Chormosomal proteins HMGN1 enhances the rate of DNA repair in chromatin EMBO J. 21: 1665-1675.
5. Zhu, N and U Hansen 2007 HMGN1 modulates estrogen mediated transcription activation through interaction with specific DNA-binding transcription factors Mol. Cell Biol. 27: 8859-8873.
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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