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Monoclonal Antibody to DDX3 (Clone IMG11F11D10) |
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| Catalog No: |
IMG-6841A |
| Contents: |
100 ug antibody in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic. |
| Isotype: |
Mouse IgG2b, Kappa |
| Clone: |
IMG11F11D10 |
| Purification: |
Protein G Chromatography was used to purify this DDX3 antibody |
| Species: |
Human |
| Host: |
Mouse |
| Application: |
Flow (I): 1 ug/10^6 cells
Western blot analysis: 2-5 ug/ml |
| Storage: |
Store DDX3 antibody at 4°C, stable for 6 months. For long-term storage, aliquot and store antibody at -20°C. |
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Background
DDX3 is a conserved RNA helicase ubiquitously expressed in the cytoplasm. A 73kD protein and member of the DEAD-box family, DDX3 appears to have important regulatory activities in a variety of cell programs including growth and cell cycle control, apoptosis, and anti-viral signaling pathways (1-3). HIV and HCV co-opt the DDX3 molecule as an essential host factor for their replication (2). Additionally DDX3 appears to recognize and sense viral RNA, interacts with IPS-1 dependent signaling pathway molecules, and TRIF, an adaptor molecule in TLR3 and TLR4 signaling pathways, as well as ZBP1 which also is involved in upregulation of IFN by HCMV (4). Loss of DDX3 may lead to enhanced proliferation and anti-apoptotic programs –pathways that promote a role for DDX3 in tumorigenesis (3). The availability of antibody specific for DDX3 provides opportunity to further detail its potential role in anti-viral immunity as well as serving as a novel target for intervention. |
Antigen
A partial recombinant protein containing amino acids 24-203 of human DDX3 was used as immunogen for this antibody. |
Genebank Info (Protein)
NP_001347 |
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Western blot analysis of human DDX3 using A) a partial recombinant protein (amino acids 24-203) and B) 293 lysate probed with DDX3 antibody at 2 ug/ml. Goat anti-mouse Ig HRP secondary antibody (20101) and PicoTect ECL substrate solution (10087K) were used for this test. |
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Intracellular FACS analysis of DDX3 in human MCF-7 and HeLa cells using DDX3 antibody at 1 ug/10^6 cells. |
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Reference
1. Lee, Cheng-Sing, AP Dias, M Jedrychowski, AH Patel, JLHsu, and R Reed 2008 Nucleic Acids Res 36: 4708-4718.
2. Schroder, M 2010 Biochem. Pharmacology. 79: 297-306.
3. Chang, P-C, C-W Chi, G-Y Chau, F-Y Li, Y-H Tsai, J-C Wu and Y-H Wu Lee 2006 ONCOGENE 23: 1991-2003.
4. Schroder, M 2011 Biochem. Soc.Trans. 39: 679-683.
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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